中国水产科学2017，Vol.24Issue(2)：268-277,10.DOI:10.3724/SP.J.1118.2017.16161
大豆浓缩蛋白替代鱼粉对大黄鱼幼鱼生长、体成分、血清生化指标及肝组织学的影响
Effect of replacing fish meal with soybean protein concentrate on growth, body composition, serum biochemical indices, and liver histology of juvenile large yellow croaker (Larimichthys crocea)
摘要
Abstract
A 56-d feeding experiment was conducted to investigate the effects of replacing fish meal in the diet with soy protein concentrate (SPC) on growth, body composition, serum biochemical indices, and liver histology of juvenile large yellow croaker,Larimichthys crocea(initial weight 10.50 g±0.04 g). The basal diet contained 40% fish meal, and five isonitrogenous (crude protein 45%) and isolipidic (crude lipid 10%) diets were formulated by replacing 0%, 25%, 50%,75%, and 100% of the fish meal with SPC and were designated the FM, R25, R50, R75, and R100 groups, respectively. All diets were supplemented with the appropriate amounts of crystalline ly-sine and methionine, based on lysine and methionine contents in the control group. Each diet was assigned ran-domly to triplicate groups of 60 ind/cage. All larvae were stocked into experimental cages (1.5 m×1.5 m×2 m) at Xihugang, Xiangshan County, Ningbo city, Zhejiang Province, China. As results, none of the experimental diets had a significant effect on the weight gain rate, specific growth rate, survival rate, or feed conversion ratio com-pared with those of fish fed the FM diet (P>0.05). Whole-body, muscle, and crude lipid contents decreased as the SPC replacement level was increased, and fish fed the R100 diet had the lowest contents. However, whole-body and muscle moisture contents increased as the SPC replacement level was increased. No differences in any of the serum biochemical indices were observed among the groups (P>0.05); briefly, serum total protein and albumin contents decreased, and fish fed the R100 diet had the lowest contents. Cholesterol and triglycerides tended to increase initially, and then decreased. Liver histology revealed serious damage to hepatocytes when SPC reached 75% (R75), as large cytoplasmic vacuole-like spaces were present, indicating excess fat accumulation, and nuclei had dissolved gradually or were absent in many of these cells. These results suggest that protein from SPC can substitute for up to 75% of the fish meal protein in the diet without affecting growth ofL. crocea.关键词
大黄鱼/大豆浓缩蛋白/生长及体成分/血清生化指标/肝组织学/鱼粉替代Key words
large yellow croaker (Larimichthys crocea)/soybean protein concentrate/growth and body composi-tion/serum biochemical indices/liver histology/fish meal replacement分类
农业科技引用本文复制引用
冯建,王萍,何娇娇,娄宇栋,党慧,邓蓉..大豆浓缩蛋白替代鱼粉对大黄鱼幼鱼生长、体成分、血清生化指标及肝组织学的影响[J].中国水产科学,2017,24(2):268-277,10.基金项目
国家自然科学基金项目(31602205) （31602205）
浙江省自然科学重点基金项目(Z16E090006) （Z16E090006）
国家海洋公益性行业科研专项(201505025) （201505025）
舟山市海洋专项(2015C41001) （2015C41001）
浙江省重中之重学科开放基金项目(XKZSC1406) （XKZSC1406）
浙江海洋大学省重中之重学科项目(20160105). （20160105）
