中国水产科学2017，Vol.24Issue(2)：414-423,10.DOI:10.3724/SP.J.1118.2017.16234
鱼类生殖细胞移植技术的发展及应用前景
Development and application prospects of fish germ cell transplantation technology
摘要
Abstract
This review introduces the development and applications of germ cell transplantation (GTC) (also called as surrogate production) as a newly emerging biotechnology. It is achieved by inducing germ-line chimerism be-tween different species. Primordial germ cells (PGCs) and spermatogonial (or oogonia) are the key material of this technique. During the last decade, several different approaches have been developed for germ cell transplantation in fish, using different type of germ cell and recipients of various ages and life stages, such as blastula-stage em-bryos, newly hatched larvae and sexually mature specimens. All of these achievements give it advantages for ap-plication in many aquaculture research fields. (1) It has opened up new scenarios for the study of germ cell and niche biology and the possibility of generating transgenic fish. (2) Combined with the technique of cryopreserva-tion of germ cell, whole testes and even whole fish, it could be a powerful tool for preserving valuable fish strains with desirable genetic traits and endangered species. (3) It presents a unique sex-selective breeding system that is superior to traditional endocrine sex control strategies. (4) It could serve as a novel and efficient method of pro-ducing fish seeds with increased genetic diversity for use in stock enhancement.关键词
鱼类生殖细胞/原始生殖细胞/精原细胞/小生境/低温保存Key words
fish germ cell/primordial germ cells/spermatogonial/niche/cryopreservation分类
农业科技引用本文复制引用
赵雅贤,周勤,于清海,夏连军,任玉芹,林听听..鱼类生殖细胞移植技术的发展及应用前景[J].中国水产科学,2017,24(2):414-423,10.基金项目
国家"十二五"科技支撑项目(2012BAD26B01) （2012BAD26B01）
农业部"引进国际先进农业科学技术"项目(2015-Z019). （2015-Z019）
