中国水稻科学2017，Vol.31Issue(2)：133-148,16.DOI:10.16819/j.1001-7216.2017.6115
干湿交替诱导水稻根表铁膜形成的基因表达谱分析
Gene Expression Profile Analysis for Alternate Wetting and Drying Induced Formation of Iron Plaque on Root Surface of Rice Seedlings
摘要
Abstract
[Objective]Alternate wetting and drying (AWD) could enhance root activity and resistance to environmental stresses, which is the most important management in rice cultivation. AWD could induce the formation of iron plaque on root surface of rice seedlings significantly. However, differentially expressed genes (DEG) of rice roots remains unknown in the process of AWD induced formation of iron plaque.[Method]In this study, DEG of rice roots were investigated in response to waterlogging (CK), AWD, CK+Fe, AWD+Fe treatments in sand-culture experiments.[Results]Compared with CK, AWD induced up-regulated expression of 506 DEG and down-regulated expression of 687 DEG. Compared with CK+Fe, AWD+Fe induced up-regulated expression of 308 DEG and down-regulated expression of 179 DEG. Compared with CK, CK+Fe induced up-regulated expression of 728 DEG and down-regulated expression of 1175 DEG. Compared with AWD, AWD+Fe induced up-regulated expression of 1252 DEG and down-regulated expression of 1189 DEG. Results from Venn diagram analysis indicated that 3822 DEG were involved in the process of AWD-induced formation of iron plaque on root surface. Gene Ontology(GO) analysis showed that a total of 270 DEG participated in the oxidation-reduction process, and 165 DEG were related to the functions of redox enzymes. Kyoto encyclopedia of genes and genomes (KEGG) results indicated that DEG involved in organelle class, signal stimulus, photosynthesis, metabolism and synthesisetc. participated the regulatory process of AWD induced formation of iron plaque on root surface. Thirty eight shared genes showed differential expression in process of both AWD and the formation of iron plaque. The proteins encoded by these genes were responsible for the resistance to disease, drought, redox, translocation and metabolism of rice. In comparison to CK, AWD induced 102 DEG in the process of oxidation and reduction, which accounted for 8.25% of the total. AWD enhanced up-regulated expression of genes that were associated with peroxidase, fatty acid oxidase, glycolic oxidaseetc. In comparison to CK, AWD raised root activity and amount of iron plaque on root surface by 22.9% and 45.7%, respectively. Results of real-time RT-PCR confirmed the transcriptional group analysis with the correlation coefficient 0.90.[Conclusion] AWD induced expression of many differential genes, enhanced root oxidative capacity, and formed iron plaque on root surface. Peroxidase, fatty acid oxidase and glycolic oxidase might be important genes involved in AWD-induced formation of iron plaque on root surface of rice seedlings.关键词
水稻/干湿交替/铁膜/差异表达基因/氧化还原相关基因Key words
rice seedling/alternate wetting and drying (AWD)/iron plaque/differentially expressed gene (DEG)/oxidation-reduction related genes分类
农业科技引用本文复制引用
傅友强,于晓莉,杨旭健,沈宏..干湿交替诱导水稻根表铁膜形成的基因表达谱分析[J].中国水稻科学,2017,31(2):133-148,16.基金项目
国家自然科学基金资助项目(31372125) （31372125）
广州市科技计划资助项目(2014J4100240). （2014J4100240）
