中国水稻科学2017，Vol.31Issue(2)：149-156,8.DOI:10.16819/j.1001-7216.2017.6137
水稻叶片内卷突变体rl(t)的鉴定与基因定位
Identification and Gene Mapping of a Rolled Leaf Mutantrl(t)in Rice
摘要
Abstract
[Ob]jectiveRice leaf is an important factor for ideal plant architecture in rice, and moderate rolling of the leaves can improve light acceptance andphotosynthetic rate. Genetic analysis and primary mapping of the target genes are useful in map-based cloning and function analysis.[Method]A stable inherited mutant, derived from anindica maintainer line Yixiang 1B by EMS treatment, exhibited significantly inward-rolling, more erect and green leaves. At the maturity stage, 10 plants ofrl(t)and its wild type(WT) were randomly selected to measure the main agronomic traits including leaf rolling index and leaf erect index. At the tillering stage, the same part of leaf ofrl16(t)and WT was taken and fixed by FAA for paraffin sectioning. The chlorophyll content inrl16(t)and WT was tested at the same stage. At the heading stage, the photosynthetic rate, intercellular carbon dioxide concentration, stamatal conductance,and transpiration rate of flag leaves in rl15(t)and WT were measured by using the portable gas exchange system Li-6400.[Result]Compared to wide type,rl(t) displayed reduced plant height, shortened panicle length as well as other agronomic traits. Leaf photosynthetic pigment contents were significantly higher than those of the wild type. However, no difference is observed in photosynthetic efficiency. Cytological analysis showed that the rolled leaf phenotype was possibly caused by the reduced size of bulliform cells. Genetic analysis indicated that rolled leaf phenotype inrl(t)was controlled by a single recessive nuclear gene. In an F2 population derived from a cross between the mutant and Nipponbare, RL(t)was mapped to a 610 kb region between Ind3 and Ind4 on the long arm of chromosome 7. In target region, there are 102 genes altogether.[Conclusion]The rolled leaf ofrl(t)was due to increased area of bulliform cells in adaxial layer and no gene related to roll-leaf was reported in the target region. So,RL(t) gene would be a putative novel rolled leaf gene.关键词
水稻/卷叶/基因定位Key words
rice/rolled leaf/gene mapping分类
生物科学引用本文复制引用
韩保林,吴先军,陶宇,张洪凯,顾朝剑,廖泳祥,彭永彬,张红宇,徐培洲,陈晓琼..水稻叶片内卷突变体rl(t)的鉴定与基因定位[J].中国水稻科学,2017,31(2):149-156,8.基金项目
国家重点研发计划资助项目(2016YFD0100406) （2016YFD0100406）
农业部公益性行业(农业)科研专项(201403002-3) （农业）
四川省农作物育种攻关项目. （）
