中国水稻科学2017，Vol.31Issue(2)：157-165,9.DOI:10.16819/j.1001-7216.2017.6130
水稻脆秆突变体bc1-wu3的鉴定与基因克隆
Identification and Gene Cloning of the Brittle Culm Mutantbc1-wu3 in Rice
摘要
Abstract
[Objective]Culm strength, an important agronomic trait, is related to lodging resistance of rice. The research of brittle culm mutants and genes has great significance to demonstrate the regulation of genetic mechanism of culm mechanical strength and breed lodging resistance varieties.[Method]In this study, a brittle culm mutant, named bc1-wu3(brittle culm 1 from Wuyujing 3), was obtained from thejaponicavarietyWuyujing 3 after60Co-γinduced mutagenesis. F2 population derived frombc1-wu3/Kasalath was used to mapping this corresponding gene by position cloning approach.[Result]The mutant was characterized by the brittle leaf, leaf sheath, and culm during the whole growing stage. Compared with the wild type, the panicle length and grain length ofbc1-wu3decreased significantly, and the grain width presented a significant increase. There was a significant decrease in the cellulose content in culm, the number of sclerenchyma cell layers and the thickness of sclerenchyma cell wall, but the contents of xylose, glucose and arabinose increased significantly inbc1-wu3. Genetic analysis showed that the brittle trait inbc1-wu3 was controlled by one single recessive nuclear gene, and the locus was mapped to a 57 kb genomic region on chromosome 3 between molecular markers M12 and Mk18. In the mapped region the cloned brittle culm geneBC1(LOC_Os03g30250) was included, which might be the candidate gene ofbc1-wu3. Sequence analysis revealed that there was a single-base substitution(G-T) in the second exon ofBC1gene inbc1-wu3 mutant, leading to a residue substitution from cysteine to phenylalanine. The data from real-time RT-PCR indicated that there was significant decrease in the expression ofBC1gene in the culm of the mutant.[Conclusion]Based on these results,we speculate that thebc1-wu3 gene is allelic toBC1 gene. These results would deepen our understanding for the function ofBC1and help us to clarify the genetic mechanisms of culm strength.关键词
水稻/脆秆突变体/BC1基因/遗传分析/基因定位Key words
Oryza sativaL./brittle culm mutant/BC1 gene/genetic analysis/gene mapping分类
生物科学引用本文复制引用
许作鹏,仲崇元,张丽佳,刘巧泉..水稻脆秆突变体bc1-wu3的鉴定与基因克隆[J].中国水稻科学,2017,31(2):157-165,9.基金项目
国家转基因生物新品种培育重大专项(2014ZX08009-024B) （2014ZX08009-024B）
国家自然科学基金资助项目(31561143008) （31561143008）
江苏省高校"青蓝工程"项目. （）
