中国生物防治学报2017，Vol.33Issue(2)：226-233,8.DOI:10.16409/j.cnki.2095-039x.2017.02.013
枯草芽胞杆菌R31影响巴西蕉根系活性氧产生及对枯萎病的防治效果
Inoculation Concentration of Bacillus subtilis Strain R31 Influenced the Roots Reactive Oxygen Species Generation of Banana (Musa spp.AAA Cavendish cv.Baxi) and Its Biological Control Effect on Fusarium Wilt
摘要
Abstract
The purpose of this paper is to study the effects of the inoculation concentrations of Bacillus subtilis strain R31 on banana roots reactive oxygen generation,its own roots colonization and their biological control on Fusarium wilt.The Mn-SOD activities and H2O2 concentrations of banana (Musa spp.AAA Cavendish cv.Baxi) seedlings roots were investigated followed by the inoculation with 1.0× 106,1.0× 107 or 1.0× 108 cfu/mL R31,respectively.The influence of different concentrations of H2O2 on R31 pellicle formation in MSgg medium,and the colonization of different concentrations R3 1-gfp on Baxi seedlings roots were also studied.Then,the biological control effects of different concentrations of R31 on Fusarium wilt were tested in fields.Results showed that significantly changed Mn-SOD activities and improved H2O2 concentrations of Baxi seedlings roots were observed after inoculated by 1.0 × 106 or 1.0 × 107 cfu/mL of R31,respectively.R31 inoculation at 1.0 × 10s cfu/mL could also significantly influence the Mn-SOD activities,which kept at 4-5 U/g at all tested time.However,there is no significant H2O2 burst of Baxi roots after 1.0x 108 cfu/mL R31 inoculation.The concentration of H2O2 higher than 80 or 120 μmol/mL could significantly inhibit the pellicle formation of R31 in MSgg medium with 1.0 × 105 or 1.0 × 106 cfu/mL cell density at 24 h,but significantly improve the pellicle formation with the same treatment at 72 h.In the treatments with1.0 × 107 and 1.0 × 108 cfu/mL R31,40 to 240 μmol/mL H2O2 concentrations had no influence on the pellicle formation of R31 at 24 h,but significantly improved the pellicle formation at 72 h.Green fluorescence was observed in the cuticle of Baxi seedlings roots inoculated by R31-gfp at 1.0 × 106 cfu/mL after 5 days,and those inoculated by R31 on 1.0 × 107 or 1.0 × 108 cfu/mL after 3 days.From December 2014 to May 2015,the Fusarium wilt control efficiency were 35.41％ and 72.96％,respectively,after the Baxi seedlings were irrigated by 2000 mL 2.0 × 106 and 2.0 × 107 cfu/mL R31 spores wettable powder suspension broth and water for 4 times in the 3 experiment areas set in Xuwen county of Zhanjiang City.It was showed that low inoculation concentrations of B.subtilis strain R31 could activate the Baxi roots immune system and caused reactive oxygen generation,then delay its own colonization on roots cuticle,which further influenced the biological control efficiency of fusarium wilt.关键词
香蕉枯萎病/生物防治/枯草芽胞杆菌/根系/免疫系统Key words
banana Fusarium wilt/biological control/Bacillus subtilis/root/immune system分类
农业科技引用本文复制引用
陈川雁,王燕,喻国辉,李一平,温书恒,吉亮..枯草芽胞杆菌R31影响巴西蕉根系活性氧产生及对枯萎病的防治效果[J].中国生物防治学报,2017,33(2):226-233,8.基金项目
广东省公益研究与能力建设专项(2014A020208015) （2014A020208015）
