中国生物防治学报2017，Vol.33Issue(2)：258-265,8.DOI:10.16409/j.cnki.2095-039x.2017.02.017
利用响应面法优化非致病性尖孢镰刀菌FJAT-9290固体发酵培养基
Optimization of Solid-state Fermentation Culture for Biocontrol Agent Non-pathogenic Fusarium oxysporum FJAT-9290 by Response Surface Methodology
摘要
Abstract
The medium components were optimized for solid-state fermentation of a biocontrol agent,non-pathogenic Fusarium oxysporum FJAT-9290.The agricultural by-products,such as bran or pig raising litter from microbial fermentation beds (MFB),were used as basic culture media.A single factor experiment was carried out to investigate the effects of kernel,sucrose and sodium nitrate on the conidia of the strain.Then,the process parameters were optimized by using Box-Behnken experimental design and the results were analyzed with response surface methodology (RSM).A multiple quadratic regression model with conidia as response value was established to determine the optimal medium component.The results showed that the regression model performed highly significant difference (P＜0.0001) and could be used to fit the experiment.When MFB and bran with a weight ratio of 17∶3 were used as basic culture media,the optimal additive amounts of kernel,sucrose and sodium nitrate were confirmed to be 39.14％,2.97％ and 0.30％,respectively.The average conidia of the strain could reach to 2.48 × 108 conidia/g under condition of the optimal medium component.The RSM analysis also indicated that the influence on conidia were kernel＞ sucrose ＞ sodium nitrate,and the interaction between sucrose and sodium nitrate was the most significant.关键词
非致病性尖孢镰刀菌/固体发酵/培养基优化/响应面Key words
non-pathogenic Fusarium oxysporum/solid-state fermentation/culture medium optimization/response surface methodology分类
农业科技引用本文复制引用
肖荣凤,郑梅霞,刘波,陈燕萍,朱育菁,葛慈斌..利用响应面法优化非致病性尖孢镰刀菌FJAT-9290固体发酵培养基[J].中国生物防治学报,2017,33(2):258-265,8.基金项目
福建省自然科学基金(2016J01128) （2016J01128）
福建省属公益类项目(2014R1018-5) （2014R1018-5）
福建省农业科技重大专项(2015NZ0003-1) （2015NZ0003-1）
