中国生物防治学报2017，Vol.33Issue(2)：266-272,7.DOI:10.16409/j.cnki.2095-039x.2017.02.018
酵母挥发性物质环辛四烯抑制灰霉菌的研究
Inhibitory Effect of the Yeast Volatile Compound Cyclooctatetraene on Botrytis cinerea
摘要
Abstract
In order to determine the inhibitory effect of volatile organic compounds produced by biocontrol yeast strain C410 on Botrytis cinerea,cyclooctatetraene,one of the dominant components of volatile organic compounds,was used to investigate its effect on sugar,protein,DNA,RNA,cell morphology and cell membrane permeability of B.cinerea.The results showed that when B.cinerea was treated with 16.5 μtL/L cyclooctatetraene,the growth rate,the reducing sugar content and the RNA integrity were significantly decreased,while the total sugar content was barely changed and the protein content was declined slightly.When treated with 49.5 μtL/L cyclooctatetraene,B.cinerea stopped growing,but the cell permeability was not affected and cells would not die.DNA was not destroyed by cyclooctatetraene.Therefore,the synthesis and metabolism of reducing sugars and RNA may be two important targets for cyclooctatetraene to suppress the pathogen growth and infection.关键词
环辛四烯/灰霉菌/抑制/机理Key words
cyclooctatetraene/Botrytis cinerea/inhibition/mechanism分类
农业科技引用本文复制引用
黄蓉,胡建坤,黄瑞荣..酵母挥发性物质环辛四烯抑制灰霉菌的研究[J].中国生物防治学报,2017,33(2):266-272,7.基金项目
国家自然科学基金(31201562) （31201562）
公益性行业(农业)科研专项(201303025) （农业）
江西现代农业科研协同创新专项(JXXTCX2015005-004) （JXXTCX2015005-004）
江西省现代农业蔬菜产业体系病虫害防治岗位专项(JXARS-06) （JXARS-06）
江西省重点研究计划项目(20161ACF60015) （20161ACF60015）
