中国生物防治学报2017，Vol.33Issue(2)：281-288,8.DOI:10.16409/j.cnki.2095-039x.2017.02.020
西芹醇层物对黄瓜枯萎病菌的化感作用及化感物质鉴定
Allelopathic Effects of Fresh Parsley Root Ethanol Extracts on Fusarium oxysporum f.sp.cucumberinum and Allelochemicals Identification
摘要
Abstract
In field production of cucumber Cucumis sativus,it is relatively difficult to control wilt caused by Fusarium oxysporum f.sp.cucumberinum.However,the extent of infection was reduced by crop rotation with parsley Apium graveloens.To identify inhibitory allelochemicals released into the soil by parsley crop,ethanol extracts from fresh parsley roots were examined after a repeated series of purification (four cycles) in column chromatography using a column with silicone coating.We used a bioassay that incorporated the various column fractions into PDA medium and co-cultured the plates with F.oxysporum.By measuring colony diameter and spores germination rate,we screened the best fractions in terms of allelopathic inhibition effect and identified further allelochemicals of the fourth chromatography using GC-MS.Optimal fractions (RE21101,RE2955,RE6235 and RE6865) obtained from the fourth cycle of chromatography showed considerable allelopathic effect.The allelopathic inhibition effects of the four optimal fractions on hypha growth compared with the fourth cycle tomographic control (CK4),were 54.59％,52.35％,51.55％ and 50.97％,respectively.In comparison to CK4,the contents of fusaric acid respectively were decreased to 38.09％,59.54％,36.78％ and 36.65％.Total 16 types allelochemicals,including N,N-dimethyl-1-hexadecanmine,heptadecanoic acid,1,6-methy-,methyl ester,hexadecanoic acid,2,4-dimethyl-benzo[b]quinoline,(+)-isomenthol,butylated,hydroxyl,alpha-arabinofuranoside,acetic acid octadecyl ester,9,12-octadecadienoic acid,2-hydroxy acid,cis-9-hexadecyl (carbon) acid,fluoren-2-azo-2,4-dithdroxybenzene,mannitol laurate,menthofuran,4-menthoxysalicylaldehyde,can be classified into 5 forms matters including organic acids,phenols,alcohols,esters,and nitrogenous compounds,were identified by the GC-MS analysis.The results provided the needed theoretical basis for the prevention and control Fusarium wilt of cucumber by using extracts of parsley.关键词
西芹鲜根/黄瓜枯萎病/化感作用/镰刀菌酸/化感物质Key words
parsley fresh root/cucumber wilt/allelopathy/fusaric acid/allelochemicals分类
农业科技引用本文复制引用
高晓敏,王琚钢,云兴福,包妍妍..西芹醇层物对黄瓜枯萎病菌的化感作用及化感物质鉴定[J].中国生物防治学报,2017,33(2):281-288,8.基金项目
海南省自然科学基金(317240) （317240）
国家农业科技成果转化项目(2014GB2A400716) （2014GB2A400716）
内蒙古自治区应用技术研究与开发项目(20150136) （20150136）
中国热带农业科学院南亚热带作物研究所中央级公益性科研院所基本科研业务费专项(1630062016004) （1630062016004）
