中国输血杂志2017，Vol.30Issue(2)：197-198,2.DOI:10.13303/j.cjbt.issn.1004-549x.2017.02.031
一例罕见Ael 08的血清学鉴定及基因测序
Serological identification and genetic sequencing of rare Ael08 subtype
丁琴丽 1凌止发 2赖蜜 3邹丽萍 1邱芳1
作者信息
- 1. 江西省赣南医学院第一附属医院输血科,江西 赣州 341000
- 2. 川北医学院附属医院
- 3. 江西省赣南医学院第一附属医院
- 折叠
摘要
Abstract
Objective To conduct serologic typing of the blood specimen of one patient in the Department of Orthopedics who were not in accordance with positive and negative typing,in order to discuss the significance of ABO subtype identification.Methods The application of conventional test and absorption elution test,serological method and direct sequencing of the ABO gene were conducted.Results The red blood cells of the patient contained weak A antigen.The irregular anti A1 antibody was detected in serum.Patient's ABO blood group was Ael subtype.Genotype was Ael08/O02.Conclusion The major serological features of A subtype were the marked reduction in the number of red cell antigens,weak or non-aggregation of red blood cells in reagent serum.Thus,this subtype could be mistyped as blood type O,which can bring risk to patients receiving blood transfusion.More attention should be given to the existence of ABO subtype,which can reduce clinical transfusion reaction,in order to ensure the safety of clinical blood transfusion.关键词
Ael亚型/弱抗原/不规则抗体/基因直接测序/输血安全Key words
Ael subtype/weak antigen/irregular antibody/gene sequencing/blood transfusion safety分类
医药卫生引用本文复制引用
丁琴丽,凌止发,赖蜜,邹丽萍,邱芳..一例罕见Ael 08的血清学鉴定及基因测序[J].中国输血杂志,2017,30(2):197-198,2.
