中国比较医学杂志2017，Vol.27Issue(3)：14-19,6.DOI:10.3969.j.issn.1671-7856.2017.03.003
不同浓度Aβ25-35蛋白模拟阿尔茨海默病模型学习记忆的差异
Aβ25-35 protein in different concentrations to create a rat model resembling Alzheimer's disease and the differences in their learning and memory ability
摘要
Abstract
Objective To observe the learning and memory ability of rats after injection of Aβ25-35 protein in different concentrations into the lateral ventricle assessed by Morris water maze test, and to explore the optimal concentration of Aβ25-35 in the preparation of AD model rats.Methods Male SD rats were randomly divided into sham operated group and model group.The rats of model group received Aβ25-35 injection in concentrations of 2 μg/μL, 4 μg/μL and 8 μg/μL, respectively.According to the Rat Brain Stereotaxic Atlas, 5 μL of aggregation of Aβ25-35 was injected into the right lateral ventricle to establish the AD rat model.7 days after successful modeling, Morris water maze was used to test thechanges of learning and memory ability of the rats.Results There was no significant difference in the average swimming speed between the two groups (P > 0.05).The escape latency time of rats in the model group was significantly increasedcompared with the sham group (P < 0.05).In the model group, the escape latency time of rats treated with 4 μg/μL and 8 μg/μL Aβ25-35 was significantly increased compared with the rats injected with 2 μg/μL (P < 0.05), while there was no significant difference between rats treated with 4 μg/μL and 8 μg/μL Aβ25-35 (P > 0.05).The activity time and distance of target quadrant of the rats injected with different concentration of Aβ25-35in the model group were significantly reduced compared with the sham group (P < 0.05), but no significant difference amongthe rats treated with different Aβ25-35 concentrations (P > 0.05).Compared with the sham-operated group, the number of platform-crossing of rats injected with different doses of Aβ25-35in the model group were significantly reduced (P < 0.05).In the model group, the rats treated with 4 μg/μL and 8 μg/μL was significantly reduced compared with the group with 2 μg/μL injection (P < 0.05).There was no significant difference between the rats injected with 4 μg/μL and 8 μg/μL (P > 0.05).Conclusions The recommended dose and concentration of Aβ25-35 to be injected into the unilateral ventricle to establisha rat model of Alzheimer's disease is 4 μg/μL in a volume of 5 μL.关键词
Aβ25-35/阿尔茨海默病/Morris水迷宫/行为学/学习记忆能力Key words
Aβ25-35 protein/Alzheimer's disease/Morris water maze test/Behavior/Learning and memory ability分类
医药卫生引用本文复制引用
王凯,李强,孙伟明,徐家淳,郭威,曹杨,周震..不同浓度Aβ25-35蛋白模拟阿尔茨海默病模型学习记忆的差异[J].中国比较医学杂志,2017,27(3):14-19,6.基金项目
高等学校博士学科点专项科研基金(20131210110012) （20131210110012）
国家自然基金(81273940). （81273940）
