中国比较医学杂志2017，Vol.27Issue(3)：20-24,5.DOI:10.3969.j.issn.1671-7856.2017.03.004
人IL-37b在大肠埃希菌中的表达、纯化及活性鉴定
Expression, purification and activity assay of human IL-37b in E.coli
摘要
Abstract
Objective To investigate the expression of recombinant IL-37b protein and removal of the endotoxin, and identify its biological activity.Methods The prokaryotic expression vector pET28/IL-37b was constructed and to transform Escherichia coli (E.coli) Rosetta.After induction with IPTG, the recombinant protein was purified through Ni2+-NTA gel column and identified by SDS-PAGE and Coomassie brilliant blue staining.Then, the endotoxin protein was removed and was treated with LPS-stimulated RAW 264.7 cells.The culture supernatant was collected.The expression of IL-6 was detected by ELISA and the biological activity of the protein was identified.Results The recombinant IL-37b with high purity was expressed and the endotoxin produced by prokaryotic expression was reduced, and it was identified to have good biological activity.Conclusions In this study a recombinant IL-37b protein with high biological activity is successfully obtained.关键词
IL-37b/表达/纯化/生物学活性Key words
IL-37b/Expression/Purification/Biological activity分类
医药卫生引用本文复制引用
李梦媛,韦荣飞,徐大模,杨星九,高苒..人IL-37b在大肠埃希菌中的表达、纯化及活性鉴定[J].中国比较医学杂志,2017,27(3):20-24,5.基金项目
中央级公益性科研院所基本科研业务费. （）
