中国比较医学杂志2017，Vol.27Issue(3)：52-56,5.DOI:10.3969.j.issn.1671-7856.2017.03.010
黄岑苷对实验性自身免疫性脑脊髓炎小鼠的影响
Protective effect of Baicalin on experimental autoimmune encephalomyelitis in mice
任应国 1张保朝 1贾东佩 1胡科1
作者信息
- 1. 南阳市中心医院神经内科, 河南 南阳 473000
- 折叠
摘要
Abstract
Objective To observe the effects of different concentrations of baicalin on the mouse model of experimental autoimmune encephalomyelitis (EAE) and to explore its mechanisms.Methods A mouse model of EAE was established with MOG33-55 peptide and bacillus Calmette-Guerin (BGG) vaccine with complete Freund adjuvant (CFA).At the third day after immunization, high and low doses of baicalin were administered to the mice intragastrically once a day for 20 days.The neurological function of mice was evaluated.TUNEL staining was used to detect apoptosis in the spinal cord tissue.The level of ATP in spinal cord tissue was detected by an ATP determination kit.Furthermore, the protein expressions of Bax, Bcl-2, cleaved cas-3 and cleaved cas-9 were detected by western blot, respectively.Results Baicalin improved the neurological function and delayed the onset time in EAE mice.关键词
黄岑苷/实验性自身免疫脑脊髓炎/细胞凋亡/线粒体/多发性硬化Key words
Baicalin/Experimental autoimmune encephalomyelitis/Cell apoptosis/Mitochondria/Multiple sclerosis分类
医药卫生引用本文复制引用
任应国,张保朝,贾东佩,胡科..黄岑苷对实验性自身免疫性脑脊髓炎小鼠的影响[J].中国比较医学杂志,2017,27(3):52-56,5.
