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动物笼具和垫料蒸汽灭菌条件的优化

陈霆 张丽芳 金迎春 邓巍 丛喆

中国比较医学杂志2017,Vol.27Issue(3):81-83,3.
中国比较医学杂志2017,Vol.27Issue(3):81-83,3.DOI:10.3969.j.issn.1671-7856.2017.03.015

动物笼具和垫料蒸汽灭菌条件的优化

Optimization of steam sterilization conditions for animal cages and beddings

陈霆 1张丽芳 1金迎春 1邓巍 1丛喆1

作者信息

  • 1. 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室,北京 100021
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摘要

Abstract

Objective To evaluate the autoclave sterilization effect on several kinds of animal cages and animal bedding, and provide the basis for safe sterilization operation.Methods Beddings and cages were sterilized with three different methods separately, and chemical indicator cards and biological indicator (Bacillus steatothermophilus) in (ATCC-7953) MTM pressure-steam sterilization were used to indicate the sterilization effects.Results The beddings were not completely dried when it was sterilized in whole package.The sterilization quality was unstable while the beddings were autoclaved after divided into separate cages.The best sterilization effect and dry beddings were obtained if the whole package was autoclaved at first and then divided into separate cages and sterilized again.Conclusions The results of this study demonstrate that dry beddings and best sterilization effect can be achieved by whole package autoclaved at first followed by sterilization of the bedding material divided into separate cages.

关键词

屏障设施/高压灭菌/垫料/笼具

Key words

Barrier facility/Autoclave/Bedding material/Cages

分类

医药卫生

引用本文复制引用

陈霆,张丽芳,金迎春,邓巍,丛喆..动物笼具和垫料蒸汽灭菌条件的优化[J].中国比较医学杂志,2017,27(3):81-83,3.

基金项目

十二五科技重大专项(2012ZX10001001-001, 2012ZX10001001-002) (2012ZX10001001-001, 2012ZX10001001-002)

卫生行业科研专项(课题编号:201302006) (课题编号:201302006)

中国比较医学杂志

OA北大核心CSTPCD

1671-7856

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