中国比较医学杂志2017,Vol.27Issue(3):81-83,3.DOI:10.3969.j.issn.1671-7856.2017.03.015
动物笼具和垫料蒸汽灭菌条件的优化
Optimization of steam sterilization conditions for animal cages and beddings
摘要
Abstract
Objective To evaluate the autoclave sterilization effect on several kinds of animal cages and animal bedding, and provide the basis for safe sterilization operation.Methods Beddings and cages were sterilized with three different methods separately, and chemical indicator cards and biological indicator (Bacillus steatothermophilus) in (ATCC-7953) MTM pressure-steam sterilization were used to indicate the sterilization effects.Results The beddings were not completely dried when it was sterilized in whole package.The sterilization quality was unstable while the beddings were autoclaved after divided into separate cages.The best sterilization effect and dry beddings were obtained if the whole package was autoclaved at first and then divided into separate cages and sterilized again.Conclusions The results of this study demonstrate that dry beddings and best sterilization effect can be achieved by whole package autoclaved at first followed by sterilization of the bedding material divided into separate cages.关键词
屏障设施/高压灭菌/垫料/笼具Key words
Barrier facility/Autoclave/Bedding material/Cages分类
医药卫生引用本文复制引用
陈霆,张丽芳,金迎春,邓巍,丛喆..动物笼具和垫料蒸汽灭菌条件的优化[J].中国比较医学杂志,2017,27(3):81-83,3.基金项目
十二五科技重大专项(2012ZX10001001-001, 2012ZX10001001-002) (2012ZX10001001-001, 2012ZX10001001-002)
卫生行业科研专项(课题编号:201302006) (课题编号:201302006)