中国畜牧兽医2017，Vol.44Issue(3)：619-627,9.DOI:10.16431/j.cnki.1671-7236.2017.03.001
树鼩Tssk6基因cDNA克隆、组织表达谱 及生物信息学分析
cDNA Cloning, Tissue Expression Profiles and Bioinformatical Analysis of Tssk6 Gene in Tree Shrews
摘要
Abstract
The purpose of this study was to clone the full-length cDNA of tree shrew's Tssk6 gene, to elucidate its tissue expression profiles and fundamental bioinformatical characteristics.In the present study, the full-length cDNA of tree shrew's Tssk6 gene was cloned with specific primers on the basis of predicted mRNA sequence of tree shrew's Tssk6 gene in GenBank, the mRNA expression of Tssk6 gene in various tree shrew's tissues was investigated via Real-time PCR.In addition, the bioinformatics on nucleotide sequence of CDS region of Tssk6 cDNA in tree shrew as well as putative protein structure had been analyzed.The results showed that: The CDS region of tree shrew's Tssk6 cDNA contained 822 bp encoding 273 amino acids;Tssk6 gene specifically expressed in tree shrew's testis;Based on the molecular phylogenetic tree constructed with CDS of Tssk6 cDNA among tree shrew and other eight mammalian species, it was inferred that tree shrew was genetically close to primates like human, chimpanzee and rhesus monkey, while it was genetically far to rodents like mouse, rat and golden hamster.This study would lay a foundation for the further study of Tssk6 gene function in tree shrew.关键词
树鼩/Tssk6基因/cDNA/克隆/生物信息学/实时荧光定量PCRKey words
tree shrew/Tssk6 gene/cDNA/cloning/bioinformatics/Real-time PCR分类
医药卫生引用本文复制引用
李元吉,杨明华,刘芳,朱方方,和绍禹,匡海鸥,李亚辉..树鼩Tssk6基因cDNA克隆、组织表达谱 及生物信息学分析[J].中国畜牧兽医,2017,44(3):619-627,9.基金项目
国家自然科学基金(31460308) （31460308）
云南中科灵长类生物医学重点实验室开放基金(云中科字2016-008号) （云中科字2016-008号）
国家自然科学基金专项基金(31040055) （31040055）
