中国畜牧兽医2017，Vol.44Issue(3)：628-634,7.DOI:10.16431/j.cnki.1671-7236.2017.03.002
陆川猪脂酰辅酶A氧化酶1基因克隆及序列分析
Cloning and Sequence Analysis of ACOX1 Gene in Luchuan Pig
摘要
Abstract
The objective of this study was to clone the acyl-coenzyme A oxidase 1 (ACOX1) gene of Luchuan pig and analyze its genetic structure with bioinformatics.A pair of special primer was designed according to predicted sequence of pig ACOX1 gene in GenBank.The ACOX1 gene was amplified by RT-PCR, the physicochemical property and secondary structure of ACOX1 gene were systemically analyzed by bioinformatics techniques.The results showed that ACOX1 gene fragment included an 1 986 bp whole length CDS (coding 661 bp amino acids).The sequence multi-aligned results showed that Luchuan pig shared 99.5%, 85.5%,87.1%,66.3%,75.6%,84.0%,82.3%,81.1% and 69.1% of similar nucleotide sequence with that of pig,human, zebrafish, chicken, rhesus monkeys, mice, rat and frog, respectively.The prediction of ACOX1 secondary structure showed that Luchuan pig ACOX1 had no signal peptide and transmembrane structure.This study suggested that the whole CDS sequence of ACOX1 gene was successfully cloned in Luchuan pig, and the cloning and analysis of ACOX1 gene provided an important foundation for further studying on the fatty deposition and lipornetabolism of ACOX1 gene in Luchuan pig.关键词
陆川猪/ACOX1基因/克隆Key words
Luchuan pig/ACOX1 gene/cloning分类
生物科学引用本文复制引用
关志惠,陈宝剑,潘天彪,覃兆鲜,刘嘉琪,马青艳,农素群,谢炳坤..陆川猪脂酰辅酶A氧化酶1基因克隆及序列分析[J].中国畜牧兽医,2017,44(3):628-634,7.基金项目
国家自然科学基金(31360544) （31360544）
广西壮族自治区畜牧研究所基本科研业务费项目(桂牧研科2015-03) （桂牧研科2015-03）
广西家畜遗传改良重点实验室自主研究课题(2015GXKLLGI-06) （2015GXKLLGI-06）
