中国畜牧兽医2017，Vol.44Issue(3)：667-672,6.DOI:10.16431/j.cnki.1671-7236.2017.03.007
ST细胞酵母双杂交cDNA文库的构建及鉴定
Construction and Characterization of the Yeast Two-hybrid cDNA Library of ST Cells
摘要
Abstract
To seek out proteins interact with VP2 structural protein of porcine parvovirus (PPV) in ST cell and study the molecular mechanisms of viral infection and pathogenicity, a ST cDNA yeast hybrid library was created.Firtly,the total RNA of ST was extracted using RNeasy Min Kit,and double strands cDNA was synthesized by SMART technique.Then, the ST cDNA library was successfully created by homologous recombination in yeast, the titer was 8.1×108 CFU/mL, the inserts varied from 250 to 1 000 bp,the average of inserted fragments was about 500 bp,and the recombination rate was 96%.These datas indicated that the yeast two-hybrid cDNA library was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PPV.关键词
猪细小病毒/酵母双杂交/ST细胞/cDNA文库Key words
porcine parvovirus (PPV)/yeast two-hybird/ST cell/cDNA library分类
生物科学引用本文复制引用
王鑫,石达,董慧,曹丽艳,陈建飞,时洪艳,张鑫,冯力..ST细胞酵母双杂交cDNA文库的构建及鉴定[J].中国畜牧兽医,2017,44(3):667-672,6.基金项目
黑龙江省科研机构创新能力提升专项(YC2015D011) （YC2015D011）
"十二五"农村领域国家科技计划课题(2015BAD12B02) （2015BAD12B02）
