中国畜牧兽医2017，Vol.44Issue(3)：673-678,6.DOI:10.16431/j.cnki.1671-7236.2017.03.008
利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA
Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray
摘要
Abstract
To determine the roles of miRNA in the developmental stages of Xiang pig,we investigated the miRNA expression patterns in different months old of Xiang pigs.The total RNA from 3 and 7 months old of Xiang pigs were collected separately.Then,the total RNA were hybridized with the miRNA microarray and the threshold (fold-change>2, P<0.05) were used to identify the different expression miRNA.The quantitative Real-time PCR was performed to verify the microarray result.Compared to the 3 months old group,there were 11 miRNA down regulated and 9 miRNA were up regulated in the 7 months old group.The lowest down regulated miRNA was miR-27b whereas the highest up regulated one was miR-155.The result of quantitative Real-time PCR consistented with the microarray result.The result suggested that some of the dysregulated expression of miRNA might participate in the lipid metabolism in hepatic cells.关键词
miRNA表达谱/香猪/肝脏Key words
miRNA expression profile/Xiang pig/liver分类
农业科技引用本文复制引用
李升,许亚楠,牛熙,冉雪琴..利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA[J].中国畜牧兽医,2017,44(3):673-678,6.基金项目
国家自然科学基金(31401091) （31401091）
贵州省科学技术基金(黔科合J字[2013]2100号) （黔科合J字[2013]2100号）
