中国畜牧兽医2017，Vol.44Issue(3)：692-697,6.DOI:10.16431/j.cnki.1671-7236.2017.03.011
肉鸡鸡胚肝脏中脂滴的发育研究
Study on Development of Broiler Liver Lipid Droplet during Embryonic Period
摘要
Abstract
This study was aimed to explore the developmental change of lipid droplet in liver of chicken embryos.120 fertilized eggs were incubated, and 6 embryos were selected randomly every day and their livers were removed for measuring the formation and development of lipid droplets from E7 to E21 during the incubation.The methods of frozen section and Sudan Ⅲ staining were used.The results showed that: ①Lipid droplets emerged early on the 9th day of chicken embryos incubation (E9).The quantities and sizes of lipid droplets became more and larger with the increasing of chicken embryos age.②Quantities of lipid droplets in every hepatic lobule decreased from central veins to the margin of hepatic lobule along hepatic cords before E18, while more lipid droplets distributed at the margin of the liver than that in the center of liver after E18.③The quantities and sizes of hepatic lipid droplets were significant difference during the three stage of E9-E14, E15-E17 and E18-E21 (P<0.05).The average sizes of hepatic lipid droplets were 3.2, 5.7 and 10.8 μm, respectively, and their average numbers were 67,327 and 397 n/mm2, respectively.In conclusion, the quantities and sizes of hepatic lipid droplets became more and larger with the increasing of chicken embryos age.关键词
肉鸡鸡胚/脂滴/肝脏/发育Key words
chicken embryos/lipid droplet/liver/development分类
农业科技引用本文复制引用
余燕,马艺源,吴世秀,陶立奎,张艳红,徐之勇,马金友..肉鸡鸡胚肝脏中脂滴的发育研究[J].中国畜牧兽医,2017,44(3):692-697,6.基金项目
河南省高校科技创新人才支持计划(13HASTIT006) （13HASTIT006）
河南省科技攻关项目(162102110038) （162102110038）
河南省教育厅自然科学基础研究项目(14A230011) （14A230011）
