中国畜牧兽医2017，Vol.44Issue(3)：717-723,7.DOI:10.16431/j.cnki.1671-7236.2017.03.014
N-羟甲基蛋氨酸钙水平对泌乳期奶牛血清含氮物和游离氨基酸的影响
Effects of N-hydroxymethyl Methionine Calcium Supplementation Levels on Serum Nitrogenous Compounds and Free Amino Acids of Lactating Dairy Cows
摘要
Abstract
To study the effects of N-hydroxymethyl methionine calcium (N-HMM-Ca) supplementation levels on serum nitrogenous compounds and free amino acids of lactating dairy cows.30 lactating Holstein dairy cows with similar parities,milk yield and days in milk were randomly assigned into 5 groups.The control group was fed with basal diet,test groups were fed with basal diet supplemented with 0.15%,0.30%,0.75% and 1.50% N-HMM-Ca,respectively.The experiment was lasted for 56 d,including 14 d of adaptation period and 42 d of experimental period.The results showed as follows:①Comparing with control group,the total protein,albumin and globulin in experimental groups were not significantly affected (P>0.05).On the 14th day,serum creatinine concentration in 1.5% level group was significantly lower than control group (P<0.05);On the 42th day,urea nitrogen concentration in 1.5% level group was significantly lower than control group (P<0.05),while the concentration of uric acid in 0.75% level group was extremely significantly higher than that in other groups (P<0.01).②On the 14th day,the concentration of serum Glu,Ser,Arg,Leu,Ile,Lys and total free amino acids in 0.15% level group was highest,which were significantly or extremely significantly higher than control group (P<0.05;P<0.01).On the 42th day,serum Arg,Cys and the total free amino acids in 0.15% level group were the highest.And serum Glu,Ser,Tyr,Met,Val and Lys in 0.30% level group were highest,which were significantly or extremely significantly higher than the control group (P<0.05;P<0.01).In summary, adding 0.15% or 0.30% N-HMM-Ca on diet of lactating dairy had no significant effect on serum nitrogen content (P>0.05),but adding 0.15% N-HMM-Ca could increase the concentration of serum free amino acids.Considering all the facts,the recommended amount of N-HMM-Ca on diet of lactating dairy cows was 0.15%.关键词
泌乳期奶牛/N-羟甲基蛋氨酸钙/含氮物/游离氨基酸Key words
lactating dairy cows/N-HMM-Ca/nitrogenous compounds/free amino acids分类
农业科技引用本文复制引用
方伟,马婷婷,陈志远,占今舜,李洪根,霍永久,赵国琦..N-羟甲基蛋氨酸钙水平对泌乳期奶牛血清含氮物和游离氨基酸的影响[J].中国畜牧兽医,2017,44(3):717-723,7.基金项目
江苏省高校优势学科建设工程项目(PAPD) （PAPD）
农业部农产品质量安全监管项目(2014) （2014）
长三角地区生鲜乳质量安全追溯体系关键技术研究应用项目(14395810100) （14395810100）
