中国畜牧兽医2017，Vol.44Issue(3)：724-731,8.DOI:10.16431/j.cnki.1671-7236.2017.03.015
枯草芽孢杆菌发酵中药制剂对鲤鱼生长性能、生化指标、抗氧化指标及抗感染能力的影响
Effect of Fermented Chinese Medicine Preparation by Bacillus subtilis on Growth Performance,Biochemical Indices, Antioxidant Parameters and Ability of Anti-infection of Common Carp
摘要
Abstract
The objective of this study was to evaluate of the growth performance, serum biochemical indices, liver antioxidant parameters and ability of anti-infection with Aeromonas hydrophila by adding the fermented Chinese medicine by Bacillus Subtilis to the carp fish feed, and to find out the appropriate supplemental level of fermented Chinese medicine in diets.Carp fingerling with an initial body weight of (35±0.5) g were set up to 6 experimental diets supplemented with 0 (control group), 0.1‰, 0.5‰, 1‰, 2‰ and 3‰ fermented Chinese medicine for 56 days, respectively.Each group had 30 fish and set up 3 repetitions.After the feeding trail,the fish were challenged with Aeromonas hydrophila to calculate the relative percentage survival (RPS).The results showed as follows: Supplementing fermented traditional Chinese medicine by Bacillus subtilis, the weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), the activities of lysozyme (LSZ), complement 3 (C3),total protein (TP),total anti-oxidation capacity (T-AOC),total superoxide dismutase (T-SOD) and catalase (CAT) in serum of common carp were enhanced at different degrees, while the feed coefficient (FCR)and malondialdehyde (MDA) in serum were declined at different degrees.When the fermented Chinese medicine supplemental levels were 2‰ and 3‰,the effects were significant (P<0.05)(except for C3, ALP and Cr).The experimental results of the virulence of common carp infected with pathogen Aeromonas hydrophila showed that,the fermented herbal could obviously improve the ability of anti-infection, the immune protective rate of the group with 2‰, 3‰ doses of the fermented Chinese medicine could reach 77.23% and 74.63%, respectively.From what had been discussed above, the group with 2‰ and 3‰ doses of the fermented Chinese medicine had significant effect on the growth performance, serum biochemical indices, liver antioxidant parameters and Aeromonas hydrophila anti-infection ability.Considering the cost of adding fermented Chinese medicine, the recommendations for the best additive quantity was 2‰.关键词
枯草芽孢杆菌/发酵中药/鲤鱼/生长性能/生化指标/抗氧化指标/抗感染能力Key words
Bacillus subtilis/fermented Chinese medicine/common carp/growth performance/biochemical indices/antioxidant parameters/ability against infection分类
农业科技引用本文复制引用
赵倩,陈玉春,高绪娜,郭婷婷,王冰,谷巍..枯草芽孢杆菌发酵中药制剂对鲤鱼生长性能、生化指标、抗氧化指标及抗感染能力的影响[J].中国畜牧兽医,2017,44(3):724-731,8.基金项目
863课题(新型抗感染功能型微生态制剂研制与应用,2013AA102806) （新型抗感染功能型微生态制剂研制与应用,2013AA102806）
