中国畜牧兽医2017，Vol.44Issue(3)：773-781,9.DOI:10.16431/j.cnki.1671-7236.2017.03.022
陆川猪MC1R和KIT基因型鉴定
Characterization of MC1R and KIT Genotypes in Luchuan Pig Population
摘要
Abstract
In order to assess the purity of Luchuan pig populations, four South Chinese local pig breeds including Putian Black pig, Yuedong Black pig, Dahuabai pig, Bama miniature pig and three foreign pig breeds including Duroc pig, Yorkshire pig and Landrace pig were studied as controls by sequencing of PCR products, MC1R and KIT genotypes in 56 Luchuan pigs were analyzed in this study.Sequencing results indicated that a splicing mutation (G>A) was presented in the first base in intron 17 of KIT gene in both Yorkshire pig and Landrace pig, in contrast, the wildtype GG of KIT gene was presented in Luchuan pig, four south Chinese local pig breeds and Duroc pig.Compared with Hainan wild boar, South Chinese local pig breeds had two missense mutations 95Val>Met and 102Leu>Pro in the coding region of MC1R gene;Compared with Yorkshire pig, Landrace pig and Duroc pig, South Chinese local pig breeds had 5 to 6 SNPs in MC1R gene 5′UTR, and in addtion, an A base deletion in MC1R gene 3′UTR.Furthermore, we found one litter of Luchuan pig with abnormal coat color.The results showed that the presentation of two distinct MC1R genotypes ED1 and Ep in both litters and the sow,but only ED1 in the boar.Considering Ep was derived from Pietrain pig, we preliminarily considered that the genome of the sow might be infiltrated with foreign pig breeds.In summary, we detected the genotypes of the coat color genes KIT and MC1R in eight pig breeds, confirmed the molecular differences of coat color between Chinese local pig breeds and foreign pig breeds, which could be useful for the further investigation of the molecular mechanism of pig coat color.关键词
陆川猪/毛色/KIT基因/MC1R基因Key words
Luchuan pig/coat color/KIT gene/MC1R gene分类
农业科技引用本文复制引用
秦珂,黄翔,曾检华,王晨,孙冠杰,宋德清,陈瑶生,刘小红,何祖勇..陆川猪MC1R和KIT基因型鉴定[J].中国畜牧兽医,2017,44(3):773-781,9.基金项目
科技基础性工作专项(2014FY120800) （2014FY120800）
广东省省级科技计划(2014B020202001) （2014B020202001）
广东省扬帆计划(2014YT02H042) （2014YT02H042）
广东省自然科学基金(2016A030313310) （2016A030313310）
国家转基因生物新品种培育重大专项(2016ZX08006003-006) （2016ZX08006003-006）
