中国畜牧兽医2017，Vol.44Issue(3)：782-789,8.DOI:10.16431/j.cnki.1671-7236.2017.03.023
牦牛PLIN2基因的克隆及其在卵巢不同发情阶段的表达
Cloning of PLIN2 Gene of Yak and Its Expression in Ovary during Different Estrus Periods
摘要
Abstract
The purpose of this study was to research the sequence characteristics and the expression of PLIN2 gene, and to analyze the relationship between the expression and the ovaries in different estrus periods.According to the gene sequence of bovine in GenBank, the primer was designed.The PLIN2 gene of yak was cloned by RT-PCR, and the expression quantity of the PLIN2 gene in different tissues of yak was detected by the semiquantitative RT-PCR.Meanwhile,the differential expression of the PLIN2 gene in different ovarian estrus periods of yak was detected by quantitative Real-time PCR.The results showed that the length of the PLIN2 gene was 1 353 bp in CDS, encoding 450 amino acids.The PLIN2 protein was predicted to be non-seretory and lacking cross-membrane domain.It was 99.4% homology identity to that of corresponding cDNA from bovine, which showed that the PLIN2 gene was quite conservative in the process of evolution.The expession profile of PLIN2 was wide in yak tissues, but there were some differences in different tissues.The expression quality was higher in kidney, breast and ovary, while it was almost nothing in lung.The result of quantitative Real-time PCR indicated that yak PLIN2 gene was expressed in different ovarian estrus periods, and the highest expression during the luteal phase, which indicated the PLIN2 gene played an essential role in yak estrous cycles.关键词
牦牛/PLIN2基因/克隆/组织表达/实时荧光定量PCRKey words
yak/PLIN2 gene/clone/expression in tissue/quantitative Real-time PCR分类
生物科学引用本文复制引用
蔡雯祎,陈通,石仙,陈伟明,胡嘉嘉,熊显荣,兰道亮,李键..牦牛PLIN2基因的克隆及其在卵巢不同发情阶段的表达[J].中国畜牧兽医,2017,44(3):782-789,8.基金项目
四川省科技支撑计划(2014NZ0114) （2014NZ0114）
西南民族大学研究生"创新型科研项目"(CX2016SZ094) （CX2016SZ094）
