中国畜牧兽医2017，Vol.44Issue(3)：790-798,9.DOI:10.16431/j.cnki.1671-7236.2017.03.024
猪圆环病毒2型江西株的全基因组克隆和序列分析
Cloning and Sequence Analysis of Complete Genomes of Porcine Circovirus Type 2 (PCV-2) Jiangxi Strains
摘要
Abstract
In order to understand the epidemiology and evolution of PCV-2 in Jiangxi province, a pair of primers was designed according to the PCV-2 gene sequence published in GenBank.After PCR amplification, we got the whole genome sequence of 9 strains PCV-2 isolates, and the nucleotide and protein sequences were analyzed, the genetic evolutionary tree was constructed.The results showed that the complete genome of 8 out of the 9 strains were 1 767 bp in length and one strain was 1 768 bp.By analyzing the whole genome sequences of the nucleotide,the homology of nucleotide sequences of the 9 strains was 94.7% to 99.9%.Compared with other whole genome sequences of PCV-2 in GenBank, the homology was 94.3% to 99.8%.The homology of nucleotide sequences of the ORF1 of the 9 strain was 96.9% to 100.0%.ORF2 and ORF3 encoding protein amino acid sequence had some locus mutation.Phylogenetic tree analysis showed that the 9 strains could be divided into 3 genotypes,5 strains belonged to PCV-2b, 3 strains belonged to PCV-2d, and 1 strain belonged to PCV-2a.This study was helpful to monitor and control of PCV-2 in Jiangxi province.关键词
猪圆环病毒2型/全基因组/序列分析Key words
PCV-2/whole genome/sequence analysis分类
农业科技引用本文复制引用
蔡高峰,李凯,张黎,王伟松,何后军..猪圆环病毒2型江西株的全基因组克隆和序列分析[J].中国畜牧兽医,2017,44(3):790-798,9.基金项目
江西省研究生创新专项资金(YC2013-S123) （YC2013-S123）
