中国畜牧兽医2017，Vol.44Issue(3)：799-806,8.DOI:10.16431/j.cnki.1671-7236.2017.03.025
香猪FSHR基因外显子10的多态性及其与产仔数间的关联分析
Polymorphism of FSHR Gene Exon 10 and Its Relationship with the Litter Size of Xiang Pigs
摘要
Abstract
Follicle-stimulating hormone (FSH), a glycoprotein secreted from pituitary, can regulate ovarian function through the FSH receptor (FSHR).To evaluate the effects of FSHR gene on the litter size of Xiang pig population, the polymorphisms of FSHR gene exon 10 were detected by polymerase chain reaction (PCR) and sequencing methods, and the effect of polymorphisms in FSHR gene exon 10 on litter size traits in Xiang pigs was analyzed using the least square analysis.In eight candidate sites, there were two polymorphic loci were detected out from Xiang pig population, which were rs322800083 (C276T) and rs332115220 (T601C).Moreover, a new SNP novel 1 (G-92A) was discovered.Four haplotypes were constructed between rs322800083 and rs332115220 sites, in which C-T was the main haplotype (45.0%) and presented closely linkage disequilibrium (r2=0.334) in Xiang pig population.Furthermore, the haplotype C-T corresponded to the highest total number born (TNB) at the second fetus of Xiang pigs, haplotype C-C to the lowest TNB.It was notified that haplotype C-T produced 0.99 TNB of Xiang pigs more than haplotype C-C (P<0.05).And the TNB at the third fetus was also the highest in haplotype C-T, which produced 1.61 and 2.33 TNB more than haplotypes T-C and C-C (P<0.01).The genotypes of locus rs332115220 was weakly correlated with the third fetus of Xiang pig population (ρ=0.429).The results suggested that haplotype C-T could be used as a molecular marker to assist selection for the litter size of Xiang pigs.关键词
FSHR基因/多态性/香猪/分子标记Key words
FSHR gene/polymorphism/Xiang pigs/molecular marker分类
农业科技引用本文复制引用
许瑶,刘畅,牛熙,冉雪琴,王嘉福..香猪FSHR基因外显子10的多态性及其与产仔数间的关联分析[J].中国畜牧兽医,2017,44(3):799-806,8.基金项目
国家高技术研究发展计划(863计划)课题(2013AA102503) （863计划）
贵州省农业攻关项目(黔科合NY[2013]3073号) （黔科合NY[2013]3073号）
贵州省"百"层次创新型人才项目(黔科合人才2016-4012号) （黔科合人才2016-4012号）
国家自然科学基金(31672390) （31672390）
