中国畜牧兽医2017，Vol.44Issue(3)：813-818,6.DOI:10.16431/j.cnki.1671-7236.2017.03.027
边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析
Study on the Polymorphisms of IGF-ⅠR Gene Alu Ⅰ Locus and Genetic Effect on Growth and Reproduction Traits in Bian Chicken
摘要
Abstract
Single nucleotide polymorphisms (SNPs) in exon 2 of insulin-like growth factor Ⅰ receptor (IGF-ⅠR) gene were detected by PCR-RFLP in Bian chicken,which was to find the correlation markers of growth and reproduction traits in Bian chicken.The results showed that AluⅠsite displayed polymorphic, and a G→A mutation at the position of the 376 bp in exon 2 was occurred.There were three genotypes (GG, GA and AA), the genotypes frequencies were 0.687, 0.296 and 0.017, respectively, GG genotype was the dominant genotype, the frequency of G allele was 0.835, which was the dominant allele.This site was in Hardy-Weinberg equilibrium (P>0.05).Correlation analysis showed that GG genotype had significantly higher body weight than those of GA genotype at the age of 8, 14, 16 and 18 weeks (P<0.05);The GG geno type had extremely significantly higher body weight at first egg than those of GA genotype (P<0.01),but it didn't mean that this locus could be used as a genetic marker for reproduction traits in Bian chicken.This results suggested that the AluⅠsite (G→A) in exon 2 of IGF-ⅠR gene could be used as a genetic marker for growth traits in Bian chicken.关键词
边鸡/胰岛素样生长因子-Ⅰ受体基因/生长性状/繁殖性状/PCR-RFLPKey words
Bian chicken/IGF-ⅠR gene/growth traits/reproduction traits/PCR-RFLP分类
农业科技引用本文复制引用
吴鹏飞,王冬,张向前,张丽,丁馥香,张跟喜..边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析[J].中国畜牧兽医,2017,44(3):813-818,6.基金项目
山西省科技公关(20140311021-1) （20140311021-1）
国家肉鸡产业技术体(nycytx-42-G1-05) （nycytx-42-G1-05）
江苏高校优势学科建设工程 （）
扬州大学科技创新培育基金(2016CXJ069) （2016CXJ069）
