中国畜牧兽医2017，Vol.44Issue(3)：928-934,7.DOI:10.16431/j.cnki.1671-7236.2017.03.042
解淀粉芽孢杆菌SSY1株的安全性试验
Evaluation of Safety of Bacillus amyloliquefaciens SSY1
摘要
Abstract
In order to determine the safety of Bacillus amyloliquefaciens SSY1, we carried out the acute toxicity, chronic toxicity, bacterial translocation, product of metabolism and drug susceptibility test.Acute toxicity test in high dose groups (1.30×1010 CFU/mL), middle dose group(0.90×1010 CFU/mL), low dose group (0.65×1010 CFU/mL) and normal saline control group.After the acute toxicity test, the mice were sacrificed 14 days after the observation.Chronic toxicity test of each group was administered once a day for 30 d, chronic toxicity test in high dose groups (0.65×1010 CFU/mL), middle dose group (0.65×109 CFU/mL), low dose group (0.65×108 CFU/mL) and normal saline control group.The results showed that all mice were without exception phenomenon, the spirit, appetite, behavior and feces and so on, no pathological changes were found on the acute toxicity test.Chronic toxicity test of the mice were no abnormal clinical changes, and there were no pathological changes.Body weight and feed utilization rate of mice, hematology, blood biochemistry indexes and viscera coefficient difference were not significant (P>0.05);Bacillus amyloliquefaciens SSY1 strain did not occur translocation in mice, amino acid decarboxylase and indole test were negative, among the 24 kinds of commonly used drugs, the strains were sensitive to 23 kinds of drugs except the cillimycin.This experiment proved that Bacillus amyloliquefaciens SSY1 had good security.关键词
解淀粉芽孢杆菌/安全性/评估Key words
Bacillus amyloliquefaciens/safety/evaluate分类
农业科技引用本文复制引用
王岩,陈楠楠,侯美如,尹珺伊,刘宇,周庆民,秦平伟,史同瑞..解淀粉芽孢杆菌SSY1株的安全性试验[J].中国畜牧兽医,2017,44(3):928-934,7.基金项目
黑龙江科技计划项目(GC13B404) （GC13B404）
黑龙江省应用技术研究与开发计划项目(GC13B406) （GC13B406）
齐齐哈尔市科学技术计划项目(NYZD-2015004) （NYZD-2015004）
