中国药房2017，Vol.28Issue(10)：1316-1319,4.DOI:10.6039/j.issn.1001-0408.2017.10.05
玄参多糖对正常生理小鼠和免疫低下状态小鼠免疫功能的影响
Effect of Polysaccharides of Radix scrophulariae on Immune Functions in Mice under Normal Physiological and Hypoimmunical State
摘要
Abstract
OBJECTIVE:To study the effect of polysaccharides of Radix scrophulariae on immune function in mice under nor-mal physiological and hypoimmunical state. METHODS:80 mice were randomly divided into normal group,model group,treat-ment high-dose,medium-dose,low-dose groups and physiological high-dose,medium-dose,low-dose groups,10 in each group. Mice in normal group and model group were received distilled water (10 mL/kg) intragastrically,treatment high-dose,medi-um-dose,low-dose groups and physiological high-dose,medium-dose,low-dose groups were administrated 0.16,0.08,0.04 g/kg drug,ig,once a day,for 7 d. From the third day,mice in model group and treatment high-dose,medium-dose,low-dose groups were respectively received cyclophosphamide(100 mg/kg),ip,for 4 d to induce hypoimmunical model. Carbon clearance test was adopted to determine the carbon clearance indexes and organs(chest,spleen)indexes of mice. Another 80 mice were grouped with the same administration,serum half hemolytic value (HC50) was determined after compressed Mianyang red blood cell sensitiza-tion. Then another 80 mice were grouped with the same administration,1% dinitrofluorobenzene method was used to induce de-layed hypersensitivity in mice;its ear swelling was determined,as well as IL-2,IL-4,immunoglobulin G(IgG),immunoglobulin M(IgM),γ-interferon(IFN-γ)contents in serum,and the proliferation in vitro of splenic lymphocytes were detected. RESULTS:High-dose polysaccharides can promote the increasing of immune organ and carbon clearance indexes(P<0.05);enhance the inten-sity of delayed type hypersensitivity (HC50 increasing)(P<0.01) and improve the decreasing of serum hemolysin in model mice (P<0.01);promote the splenic lymphocytes proliferation and increase IL-2,IL-4,IgG,IgM,IFN-γ contents in serum(P<0.05 or P<0.01). CONCLUSIONS:Polysaccharides can enhance the immune function in mice under normal physiological and hypoim-munical state induced by cyclophosphamide.关键词
玄参多糖/免疫功能/生理状态/免疫低下状态/小鼠Key words
Polysaccharides/Immune function/Physiological state/Hypoimmunical state/Mice分类
医药卫生引用本文复制引用
李自辉,张宁,董婉茹,于卉,刘树民..玄参多糖对正常生理小鼠和免疫低下状态小鼠免疫功能的影响[J].中国药房,2017,28(10):1316-1319,4.基金项目
国家重点基础研究发展计划"973"计划(No.2013CB531804) （No.2013CB531804）
