中国药房2017，Vol.28Issue(10)：1326-1328,3.DOI:10.6039/j.issn.1001-0408.2017.10.08
淫羊藿苷对自发性高血压大鼠肾损伤的改善作用及机制研究
Study on Improvement Effect and Mechanism of Icariin on Renal Lesion in Rats with Spontaneous Hyper-tension
摘要
Abstract
OBJECTIVE:To observe the effect of pathological lesion of renal tissue in rats with spontaneous hypertension (SHR),and study its mechanisms based on nuclear factorκB(NF-κB)signaling pathway. METHODS:21 SHR were randomly di-vided into model group and ICA low-dose,high-dose groups(20,40 mg/kg,denoted by ICA-L,ICA-H groups);other 7 homolo-gous Kyoto rats (WKY) were regarded as control group. All rats were intragastrically administrated,twice a day,for 11 weeks, rats in control group and model group received equal volume of double distilled water,ig. Pathological changes in renal tissue in each group were observed;Western blot method was used to detect protein expressions of p-NF-κB-p65,IκB and TNF-α in renal tissue. RESULTS:Compared with control group,model group showed disorder renal structure,narrow and irregular glomerular cysts;the protein expression of IκB was significantly down-regulated,protein expressions of p-NF-κB-p65 and TNF-α were signifi-cantly up-regulated(P<0.01). Compared with model group,the above-mentioned changes of rats showed improvement in ICA-L, ICA-H groups;the protein expression of IκB was significantly up-regulated in ICA-L,ICA-H groups (P<0.05 or P<0.01);the protein expressions of p-NF-κB-p65 and TNF-α were significantly down-regulated(P<0.01)in ICA-H groups;p-NF-κB-p65 pro-tein expression was significantly down-regulated in ICA-L group(P<0.05);while there was no significant difference in TNF-αpro-tein expression in ICA-L group(P>0.05). CONCLUSIONS:ICA plays a role in improving renal pathological lesion in SHR,and the mechanism may be related to inhibiting NF-κB signaling pathway.关键词
淫羊藿苷/自发性高血压大鼠/肾组织/磷酸化核因子κB-p65/肿瘤坏死因子αKey words
Icariin/Spontaneously hypertension rats/Renal tissue/p-NF-κB-p65/TNF-α分类
医药卫生引用本文复制引用
朱玲,钱志强,李叶丽,王俊逸,杨华,杨丹莉..淫羊藿苷对自发性高血压大鼠肾损伤的改善作用及机制研究[J].中国药房,2017,28(10):1326-1328,3.基金项目
国家自然科学基金资助项目(No.81241142, 81660679) （No.81241142, 81660679）
