中国药房2017，Vol.28Issue(12)：1673-1676,4.DOI:10.6039/j.issn.1001-0408.2017.12.25
高产高油桂郁金种质的筛选
Screening of High-yield and High-oil Curcuma kwangsiensis
摘要
Abstract
OBJECTIVE:To establish a method for analyzing the volatile oil of high-yield and high-oil Curcuma kwangsiensis, and to provide reference for its breeding. METHODS:GC-MS was performed on the column of HP-5MS quartz elastic capillary column,carrier gas was high purity helium(99.999%),flow rate was 1.0 ml/min,inlet temperature was 250 ℃,the initial tem-perature of column was 50 ℃(temperature programmed),split injection with split ratio of 10:1. Mass spectrometry conditions:ionization mode was the electron impact ion source,ionization energy was 70 eV,the ion source temperature was 230 ℃,the quadrupole temperature was 150 ℃, transmission line temperature was 280 ℃,the electron multiplier voltage was 1588 V,and mass scanning range was m/z 45 to 500. High-yield and high-oil germplasm of were screened from 100 different germplasms,the volatile oil composition of single plant and relative percentage contents of each composition were compared,SPSS 22.0 software was used for cluster analysis. RESULTS:Totally 10 high-yield and high-oil germplasm were screened,54 kinds of compounds were identified,the common compositions of 10 different germplasms were camphor,1-caryophyllene,γ-elemene,curcumene, gemma ketone and new curdione,most germplasms contained borneol,isoborneol,δ-elemene,germacrene and calamine. The C78,C104,purpose 2,volatile oil content and relative percentage contents of active compositions in volatile oil were high. The 10 high-yield and high-oil germplasms can be divided into 3 groups. CONCLUSIONS:The study basically clears the main chemicalcomposition of volatile oil of high-yield and high-oil C. kwangsiensis,C78,C104 and purpose 2 are the more excellent strains in high-yield and high-oil C. Kwangsiensis germplasm.关键词
桂郁金/种质/挥发油成分/高产/高油Key words
Curcuma Kwangsiensis/Germplasm/Volatile oil components/High-yield/High-oil分类
医药卫生引用本文复制引用
靳雅惠,杨传强,王建..高产高油桂郁金种质的筛选[J].中国药房,2017,28(12):1673-1676,4.基金项目
国家自然科学基金资助项目(No.81160500) （No.81160500）
广西科技厅课题(No.桂科攻1099063-2) （No.桂科攻1099063-2）
广西研究生教育创新计划资助项目(No.YCSZ2015181) （No.YCSZ2015181）
