中国药理学通报2017，Vol.33Issue(4)：469-474,6.DOI:10.3969/j.issn.1001-1978.2017.04.006
别嘌醇改善果糖诱导的大鼠高尿酸血症与调节肠道葡萄糖转运子表达的关系研究
Effect of allopurinol on serum level of uric acidand intestinal expression of glucose transportersin rats with fructose-induced hyperuricemia
摘要
Abstract
Aim To investigate the effect of allopurinol and benzbromarone on serum level of uric acid, hepatic xanthine oxidase(XOD) activity and intestinal expression of glucose transporter(GLUT) 2 and 5 in rats with fructose-induced hyperuricemia.Methods Wistar rats were fed with 10% fructose in drinking water for consecutive 8 weeks to induce HUA.Treatment with 5 mg·kg-1 allopurinol or 10 mg·kg-1 benzbromarone were intragastricly administered from 5~8 weeks.Serum level of uric acid and XOD activity in liver were tested.Expression of GLUT2 and GLUT5 in intestine was analyzed by immunohistochemistry staining and Western blot.Results Treatment with allopurinol or benzbromarone significantly decreased the serum level of uric acid in fructose-induced hyperuricemic rats.At the same time, allopurinol treatment significantly reduced the XOD activity in liver and GLUT5 expression in intestine.Nevertheless, benzbromarone treatment did not show inhibitory effect on hepatic XOD activity and intestinal GLUT5 expression.In addition, neither allopurinol nor benzbromarone showed inhibitory effect on GLUT2 expression in intestine.Conclusions Allopurinol decreases serum level of uric acid in fructose-induced hyperuricemic rats.The mechanism is related to reducing XOD-mediated uric acid production in liver, and decreasing GLUT5-mediated fructose absorption in intestine.关键词
别嘌醇/果糖/尿酸/高尿酸血症/葡萄糖转运子2/葡萄糖转运子5Key words
allopurinol/fructose/uric acid/hyperuricemia/glucose transporter 2/glucose transporter 5分类
医药卫生引用本文复制引用
陈刚,贾萍..别嘌醇改善果糖诱导的大鼠高尿酸血症与调节肠道葡萄糖转运子表达的关系研究[J].中国药理学通报,2017,33(4):469-474,6.基金项目
国家自然科学基金资助项目(No 81503420) （No 81503420）
重庆市教委科学技术研究项目(No KJ1400626) （No KJ1400626）
重庆市科委前沿与应用基础研究项目(No cstc2016jcyja0475) （No cstc2016jcyja0475）
