中国药理学通报2017，Vol.33Issue(4)：480-486,7.DOI:10.3969/j.issn.1001-1978.2017.04.008
坏死性凋亡介导化学性缺氧引起的HT22海马神经元损伤和炎症
Necroptosis mediates chemical hypoxia-induced injury andinflammation in HT22 hippocampal cells
摘要
Abstract
Aim To investigate whether necroptosis mediates chemical hypoxia-induced HT22 mouse hippocampal cell injury and inflammation.Methods HT22 hippocampal cells were exposed to cobalt chloride (CoCl2) to establish a model of the chemical hypoxia-induced injury and inflammation.The expression level of RIP3 (an index of necroptosis) was determined by Western blot.Cell counter kit-8 (CCK-8) assay was used to test the cell viability.Lactate dehydrogenase (LDH) activity in the culture medium was measured with commercial kits.Mitochondrial membrane potential (MMP) was examined by rhodamine123 staining followed by photofluorography.The intracellular level of reactive oxygen species (ROS) was detected by 2', 7'-dichlorfluorescein-diacetate (DCFH-DA) staining followed by photofluorography.The secretion levels of interleukin-1β (IL-1β) and tumor necrosis factor-a (TNF-α) were measured by ELISA.Results Treatment of HT22 hippocampal cells with 600 μmol·L-1 CoCl2 for 36 h markedly induced cytotoxicity, leading to a decrease in cell viability to (52.0±2.65) % , indicating that chemical hypoxia-induced cellular injury model was successfully set up.Besides, CoCl2 induced considerable injuries and inflammation, evidenced by increases in LDH activity, ROS production, MMP loss, as well as the secretion levels of IL-1β and TNF-α.Co-treatment of the cells with 40~100 μmol·L-1 Nec-1 (a specific inhibitor of necroptosis) and CoCl2 markedly attenuated the decrease in viability induced by CoCl2, reaching the best anti-cytotoxicity inhibitory effect at 80 μmol·L-1.Meanwhile, the co-treatment with 80 μmol·L-1 Nec-1 blocked the above injuries and inflammatory response induced by CoCl2.In addition, treatment of HT22 hippocampal cells for 6~48 h up-regulated the expression of RIP3, and Nec-1 alleviated the up-regulation of RIP3 expression level induced by CoCl2.Conclusion Necroptosis mediates chemical hypoxia-induced HT22 hippocampal cell injury and inflammation.关键词
坏死性凋亡/化学性缺氧/氯化钴/HT22细胞/损伤/炎症Key words
necroptosis/chemical hypoxia/cobalt chloride/HT22 hippocampal cell/injury/inflammation分类
医药卫生引用本文复制引用
王波,徐勇,李祥,侯娇艳,周忠群,田绍文,旷昕..坏死性凋亡介导化学性缺氧引起的HT22海马神经元损伤和炎症[J].中国药理学通报,2017,33(4):480-486,7.基金项目
国家自然科学基金资助项目(No 8140050613) （No 8140050613）
湖南省教育厅资助课题(No 15C1208) （No 15C1208）
