中国药理学通报2017，Vol.33Issue(4)：487-492,6.DOI:10.3969/j.issn.1001-1978.2017.04.009
二氢槲皮素预处理对心肌缺血/再灌注损伤抗氧化作用的影响
Antioxidation effect of dihydroquercetin pretreatment in isolatedrat hearts during myocardial ischemia reperfusion injury
摘要
Abstract
Aim To investigate the protective effects of dihydroquercetin(DDQ) against myocardial ischemis reperfusion injury(MIRI) in rats.Methods Male Sprague-Dawley rats were randomly divided into 4 groups(n=10):normal,control,I/R model, and I/R model+DDQ(5,10 mg·L-1).This study used an isolated Langendorff rat heart model.The left ventricu-lar developed pressure(LVDP),heart rate(HR) and the maximum rise and fall rate of the left ventricular pressure(±dp/dtmax) were monitored and documented using a physiological recorder.The levels of lactate dehydrogenase(LDH) and creatine kinase(CK) were analyzed using enzyme-linked immunosorbent assay(ELISA).Infarct size was measured using 2,3,5-triphenyltetrazolium chloride staining.The levels of superoxide dismutase(SOD) and malondialdehyde(MDA), as well as the ratio of glutathione/glutathione disulfide(GSH/GSSG) were measured via ELISA.HE staining was used to observe the pathological changes of myocardial tissue.Results Compared with the I/R model group, the I/R model+DDQ groups raised hemodynamic parameters, SOD level, and GSH/GSSG ratio;and reduced the amount of CK, LDH, MDA levels.Moreover, the I/R model+DDQ groups had lower infarct size and pathological changes in myocardial tissue than I/R model group.Conclusion DDQ exertes cardioprotective effects against I/R via improving the oxygen free radical scavenging ability, the inhibition of oxygen free radical and reducing lipid peroxidation.关键词
二氢槲皮素/抗氧化/心肌缺血/再灌注/离体心脏/心肌梗死/氧自由基Key words
dihydroquercetin/antioxidant/ischemia/reperfusion/injury isolated heart/miocardial infarction/oxygen free radical分类
医药卫生引用本文复制引用
卢宁,韩吉春,任博雪,李德芳,王博,郝雯瑾,郑秋生..二氢槲皮素预处理对心肌缺血/再灌注损伤抗氧化作用的影响[J].中国药理学通报,2017,33(4):487-492,6.基金项目
新疆特种植物药物质基础与作用机制研究创新团队基金(No 2015BD005) （No 2015BD005）
滨州医学院高层次人才引进基金(No BY2014KYQD01) （No BY2014KYQD01）
滨州医学院科研启动基金 (No BY2014KYQD30) （No BY2014KYQD30）
