中国药理学通报2017，Vol.33Issue(4)：498-506,9.DOI:10.3969/j.issn.1001-1978.2017.04.011
高脂血症与Aβ的协同作用促进引发阿尔茨海默症
Study of synergistic effect of hyperlipoproteinemiaand Aβ in processing of Alzheimer′s disease
摘要
Abstract
Aim To research the synergistic effect of hyperlipoproteinemia and Aβ in the processing of Alzheimer′s disease.Methods Seventy SD rats were randomly divided into seven groups, and dealt with D-gal(hypodermic injection), hyperlipemia diet, microinjection into both side of CA1 section in hippocampus, independently.Morris water maze(MWM) test was used to evaluate the spatial memory impairments.Tau and tau(pThr181) pathology in the hippocampus were detected using Western blot and immunohistochemistry.Nissl′s staining was used to detect cell apoptosis.Results Aβ25-35-treated rats showed significant impairments of spatial memory in MWM test, especially in the group of D-gal+Aβ25-35+HLD(P<0.01).Furthermore, these rats treated with Aβ25-35, D-gal, and hyperlipemia diet, exhibited significantly increased phosphorylation of tau, particularly in the Thr181 site.Conclusion Hyperlipoproteinemia is the risk factor for older person, which could strengthen the toxic effect of Aβ, and promote phosphorylation of tau.关键词
高脂血症/Aβ/阿尔茨海默病/tau/过度磷酸化/衰老Key words
hyperlipoproteinemia/Aβ/Alzheimer′s disease/tau/hyperphosphorylation/aging分类
医药卫生引用本文复制引用
侯江淇,张欣,龙倩,楚世峰,郭蕾,贺文彬,张俊龙,陈乃宏..高脂血症与Aβ的协同作用促进引发阿尔茨海默症[J].中国药理学通报,2017,33(4):498-506,9.基金项目
国家自然科学基金资助项目(No 81573853,81273629) （No 81573853,81273629）
中国医学科学院医学与健康科技创新工程重大协同创新项目(创新团队项目)(No 2016I2M1004) （创新团队项目）
山西省回国留学人员科研资助项目(No 2013-134) （No 2013-134）
湖南省重点研发计划项目(No 2015SK2029-1) （No 2015SK2029-1）
