中国油料作物学报2017，Vol.39Issue(1)：1-12,12.DOI:10.7505/j.issn.1007-9084.2017.01.001
大豆转录因子GmNAC8的克隆及耐旱性功能分析
Cloning and function analysis of GmNAC8 transcription factor gene response to drought stress
摘要
Abstract
In order to obtain functional genes in response to drought stress,a DGE (digital gene expression) profile,two drought-tolerance and sensitivity materials under drought stress,had been constructed in our previous study.A gene encoding NAC tanscription factor,named as GmNAC8,was chosen as candidate gene from DGE pro-file.The gene had CDS full-length sequence (1 092bp),and encodes 363 amino acid.By software analysis,its protein had a molecular mass of 41.82kD and PI 8.51.The N-terminal aa residue of GmNAC8 contains a typical NAC conserved domain with 42 aa,and C-terminal aa residue is highly variable.Homology tree demonstrated that GmNAC8 shared high homology with Phaseolus vulgaris,Vigna angularis and Vigna radiata.Subcellular localiza-tion revealed that GmNAC8 was expressed in nucleus.Transcription analysis by qRT-PCR showed that the expres-sion of GmNAC8 in leaf was apparently higher than that in root from transgenic lines.GmNAC8 was up-regulated by 0.1 mmol/L IAA and ABA,and was down-regulated by 0.1 mmol/L GA and SA.The leaves of transgenic Ara-bidopsis thaliana lines were greener than CK,and drought tolerance was improved under water-deficient condition by overexpression of GmNAC8.Drought-tolerant physiological analysis revealed that the contents of protein,pro-line,and POD activity were higher in transgenic lines in 10 days drought treatment,while malonaldehyde (MDA) was significantly lower,only occupied 42％.This study would provide understanding of the molecular mechanisms of plants response to drought.关键词
大豆/干旱胁迫/GmNAC8基因/功能分析Key words
Glycine max/drought stress/GmNAC8 gene/function analysis分类
农业科技引用本文复制引用
方义生,单志慧,邱德珍,陈李淼,周新安,刘宝红,陈水莲,陈海峰,张婵娟,袁松丽,郝青南,杨中路,张晓娟..大豆转录因子GmNAC8的克隆及耐旱性功能分析[J].中国油料作物学报,2017,39(1):1-12,12.基金项目
抗旱转基因大豆新品种培育重大专项(2013ZX08004002-008) （2013ZX08004002-008）
现代农业产业技术体系(CARS-04) （CARS-04）
