中国油料作物学报2017，Vol.39Issue(1)：30-36,7.DOI:10.7505/j.issn.1007-9084.2017.01.005
分子标记辅助回交选育高油酸花生新种质
Development of high oleic acid peanut from molecular marker assisted selection
摘要
Abstract
To breed high oleate peanut cultivars suitable for Shandong and Huang-Huai-Hai region of Chi-na,HY23 × DF12 (SAAS-01),HY31 × KN176 (SAAS-02) and HY31 × Sunoleic95R,3 cross combinations were made using two elite peanut cultivars Huayu 23 and Huayu 31 as female parents,and 3 high oleate peanuts Sunoleic95R,DF12 and Kainong176 as male parents.One cross,4 backcrosses and 1 selfing were carried out within 3 years.The progenies of each cross were examined by CAPS (cleaved amplified polymorphic sequence)and sequencing of PCR products.KASP (kompetitive allele specific PCR) was performed for more efficient exam-ining of BC4F2 genotypes.The numbers of BC4F2 seeds with aabb genotype of three combinations SAAS-01,SAAS-02 and SAAS-03 were 1,18 and 26,respectively.Different numbers of BC4F2 seeds with Aabb,aaBb and AaBb genotypes were obtained.These seeds could be grown and selfed to obtain the aabb homozygous.These materials could provide useful resources for breeding of high oleic acid peanut cultivars with high yield and suitable for Shandong and Huang-Huai-Hai region.关键词
花生/高油酸/分子标记辅助选择/回交/育种Key words
peanut/high oleic acid/marker assisted selection/backcross/breeding分类
农业科技引用本文复制引用
赵术珍,侯蕾,李长生,赵传志,任丽,李爱芹,邓丽,夏晗,王兴军..分子标记辅助回交选育高油酸花生新种质[J].中国油料作物学报,2017,39(1):30-36,7.基金项目
玛氏集团巧克力事业部全球花生可持续发展计划 （）
国家自然科学基金(31471526) （31471526）
山东省农业良种工程 （）
山东省优秀中青年科学家科研奖励基金(BS2013SW006) （BS2013SW006）
