中国油料作物学报2017，Vol.39Issue(1)：113-116,4.DOI:10.7505/j.issn.1007-9084.2017.01.017
抗黄曲霉毒素单链抗体在毕赤酵母X-33中的表达
Expression of a single-chain Fv antibody against aflatoxin in Pichia pastoris X-33
摘要
Abstract
To reduce the cost of single-chain antibody for aflatoxin and increase the expression activity of sin-gle-chain antibody,the recombinant plasmid containing the aflatoxin scFv gene pCANTAB 5E-scFv1A7 was cloned to obtain scFv gene fragment and construct a yeast expression vector pPICZαA-scFv1A7.Then Sac Ⅰ en-zyme was linearized into Pichia pastoris X-33 chromosome genome to construct pPICZαA-scFv1A7 X-33 re-combinant yeast.Single-chain antibody was successfully expressed with 0.8％ methanol induced secretion.Indi-rect competitive ELISA method was used to detect aflatoxin B1 revealed the detection inhibition rate (IC50) of 4.5 ng/mL,which indicated that the recombinant expression product scFv had good antigen binding activity and could be applied in diagnosis of aflatoxin contamination.关键词
黄曲霉毒素/单链抗体/毕赤酵母/表达Key words
aflatoxin/scFv/Pichia pastoris/expression分类
农业科技引用本文复制引用
王婷,雷佳文,李培武,张奇,张文,何婷..抗黄曲霉毒素单链抗体在毕赤酵母X-33中的表达[J].中国油料作物学报,2017,39(1):113-116,4.基金项目
公益性农业行业科研专项(201203037) （201203037）
国家自然科学基金(31171702,31101299) （31171702,31101299）
