中国岩溶2016，Vol.35Issue(6)：614-618,5.DOI:10.11932/karst20160602
双同位素示踪定量微藻对碳源利用份额的方法研究
Application ofbidirectional labeling method to quantifying carbon utilization in microalgae
摘要
Abstract
August 2015,the Chlorella pyrenoidosawas cultivated in a greenhouse with different inorganic carbons(NaH13CO3 with different δ13C values added) in the culture medium.The δ13C of inorganic carbon in the medium and the biomass of the microalgae were detected on a daily basis.In the meantime,the organic stable carbon isotope compositions of the microalgae was also measured.The proportion of the added inorganic carbon used by microalgae was quantified by comparing their stable carbon isotope compositions using the bidirectional labeling method (NaH13CO3with different δ13 Cvalues was added).This study compared respectively both inorganic carbon and organic carbon of the stable carbon isotope compositions.The results are as follows,it is 0.19 under 5.0 mmol/L NaHCO3,0.37 under 10.0 mmol/L NaHCO3,and 0.57 under 20.0 mmol/L NaHCO3.At last,we analyzed the two methods for quantifying the carbon sources in algae.It is very important to calculate the proportion of different inorganic carbon in the carbon cycle research in karst lakes.关键词
碳酸盐利用能力/稳定碳同位素/微藻Key words
inorganic carbon utilization/stable carbon isotope/microalgae分类
农业科技引用本文复制引用
李海涛,吴沿友,赵丽华,张开艳,杭红涛..双同位素示踪定量微藻对碳源利用份额的方法研究[J].中国岩溶,2016,35(6):614-618,5.基金项目
国家自然科学基金项目(40973060) （40973060）
贵州省科学技术基金(黔科合J字[2014]2131号) （黔科合J字[2014]2131号）
