中国医学前沿杂志（电子版）2017，Vol.9Issue(2)：16-19,4.DOI:10.12037/YXQY.2017.02-04
扫描电镜观察体外冲击波对去势合并吊尾大鼠骨超微结构的影响
Using scanning electron microscopy to investigate the effect of extracorporeal shock wave on bone ultra-structure of ovariectomized and tail-suspended rats
摘要
Abstract
Objective To study the effect of extracorporeal shock wave (ESW) on rat bone microstructure when treated with ovariectomy and tail suspension. Method 184-month-old female SD rats were randomly divided into control group, ovariectomy + hindlimb unloading group and ESW group, 6 rats per group. Every rat in ovariectomy + hindlimb unloading group underwent ovariectomy as well as tail suspension. The rats in ESW group received ESW treatment after ovariectomy +hindlimb unloading. The ESW treatment (energy flux density: 3 bar, frequency: 5 Hz, 1500 pulses) was applied to the hind leg of osteoporotic rats for 4 weeks and once per week. 1 month later all of the rats were sacrificed and the left tibias were collected with soft tissue removed. Then microscopy (SEM) was used to observe the bone. Result In control group, the trabecular connected with each other closely and network structure was integrity; while in the ovariectomy +hindlimb unloading group, the trabecular network structure was completely destroyed and trabecular number was reduced significantly. And the trabecular structure in ESW group was partly destroyed. The quantity and integrity of trabecular was higher than ovariectomy +hindlimb unloading and lower than control group. Conclusion ESW can partly protect bone structure which is destroyed by ovariectomy and tail suspension.关键词
体外冲击波/扫描电镜/超微结构Key words
Extracorporeal shock wave/Scanning electron microscopy/Ultrastructure引用本文复制引用
张浩冲,刘水涛,耿欢,杨军,赖斌,邢更彦..扫描电镜观察体外冲击波对去势合并吊尾大鼠骨超微结构的影响[J].中国医学前沿杂志（电子版）,2017,9(2):16-19,4.基金项目
国家自然科学基金项目(31172169) （31172169）
