中国药业2017，Vol.26Issue(5)：6-9,4.DOI:10.3969/j.issn.1006-4931.2017.05.002
瘤果黑种草子总黄酮对RAW264.7细胞及豚鼠气管平滑肌的作用
Effect of Total Flavones from Nigella Glandulifera on RAW264. 7 Cells and Guinea Pig Tracheal Smooth Muscle
摘要
Abstract
Objective To study the effect of total flavones from Nigella Glandulifera ( TFNG ) on RAW264. 7 cells and guinea pig tracheal smooth muscle. Methods The high, medium and low dose ( 20, 40, 80 μg/mL ) of TFNG was used to detect the secretion of inter-leukin-8 in RAW264. 7 cells induced by lipopolysaccharide ( LPS ) by enzyme-linked immunosorbent assay ( ELISA ) and Griess Reagent method. IL-8, IL-6 and nitric oxide ( NO ) were measured. The tracheal spiral specimens of guinea pigs were prepared by the method of constant-temperature perfusion with tracheal trachea. The changes of the tension were observed by 1, 2, 4 g/L dose of TFNG and 10 g/L decoction on antipruritic effects of guinea pig tracheal smooth muscle induced by tacrolipotentin ( ACh ) and histamine ( His ) . Results TFNG had no significant toxic effect on RAW264. 7 cells at less than 100 μg/mL. Compared with LPS group, the TFNG low, medium and high dose group ( 20, 40, 80 μg/mL ) could inhibit RAW264. 7 cells and the levels of IL-6, IL-8 and NO ( P < 0. 05 ) , and there was a significant dose-dependent relationship. The TENG low, medium and high dose group ( 1, 2, 4 g/L ) could significantly relax the bronchial smooth muscle spasms of guinea pig induced by ACh and His, the difference was statistically significant ( P < 0. 01 ) . Conclusion TFNG has some anti-inflammatory and bronchial antispasmodic effects in vitro.关键词
瘤果黑种草子总黄酮/巨噬细胞/支气管解痉/抗炎/豚鼠Key words
total flavones from Nigella Glandulifera/macrophage/bronchodilation/anti-inflammatory/guinea pig分类
医药卫生引用本文复制引用
曹园,耿东升,孙玉华,袁凤娟..瘤果黑种草子总黄酮对RAW264.7细胞及豚鼠气管平滑肌的作用[J].中国药业,2017,26(5):6-9,4.基金项目
国家自然科学基金[ 69483382 ]. （）
