摘要
Abstract
Objective To study the antioxidant effect of total flavonoids in the fruits of Pranus cerasifera ( PCE ) . Methods The total re-ducing ability of PCE was determined by UV-Vis spectrophotometry. The total reduction ability of PCE, the effects of 1, 1-Diphenyl-2-picrylhydrazy ( DPPH ) , superoxide anion ( O2-) , hydroxyl radical ( · OH ) , nitric oxide ( NO ) and the inhibition of lipid peroxidation were determined by the method of in vitro chemical simulation. The antioxidant activity of PCE was studied. Results The absorbance of PCE increased at the concentration of 1, 2, 4, 6, 8 g/mL ( 0. 189 ± 0. 02, 0. 287 ± 0. 01, 0. 451 ± 0. 003, 0. 708 ± 0. 001, 0. 769 ± 0. 002 ) , which had strong reducing ability and could significantly inhibit the generation of ·OH, O2 -, DPPH, NO;The levels of malondialdehyde ( MDA ) in the control group, ascorbic acid group and PCE group ( 0. 40, 0. 80, 0. 160 g/L ) were 50. 1%, 19. 6% and 31. 8%, 45. 9%, respec-tively, showing the levels of MDA could effectively inhibited. Conclusion PCE has strong antioxidant effect and has obvious dose-ef-fect relationship.关键词
紫叶李果实总黄酮/脂质过氧化/氧自由基/抗氧化Key words
total flavonoids in the fruits of Pranus cerasifera ( PCE )/lipid peroxidation/oxygen free radical/antioxidant分类
医药卫生
