中国药业2017，Vol.26Issue(5)：29-31,3.DOI:10.3969/j.issn.1006-4931.2017.05.007
高效液相色谱法测定甲基泼尼松龙琥珀酸钠含量
Content Determination of Methylprednisolone Sodium Succinate by HPLC
乔晓芳1
作者信息
- 1. 河南省食品药品审评查验中心,河南郑州 450000
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the content determination of methylprednisolone sodium succinate. Methods The mobile phase consisted of acetonitrile-3% glacial acetic acid(29 :71). The detection wavelength was 254 nm, the column temperature was 25 ℃ and the flow rate was 1. 0 mL/min. The sample volume was 20 μL. Results The linear relation of the concentration and peak area was good in the range of 0. 08-0. 12 mg/mL( r=0. 9995). The average percentage of recovery was 99. 16%, the RSD was 0. 09%( n=5). Conclusion The method is accurate and reproducible, which can be used for the quality control of methylprednisolone sodium succinate.关键词
甲基泼尼松龙琥珀酸钠/高效液相色谱法/含量测定Key words
methylprednisolone sodium succinate/HPLC/content determination分类
医药卫生引用本文复制引用
乔晓芳..高效液相色谱法测定甲基泼尼松龙琥珀酸钠含量[J].中国药业,2017,26(5):29-31,3.
