中国药业2017，Vol.26Issue(5)：32-34,3.DOI:10.3969/j.issn.1006-4931.2017.05.008
高效液相色谱法测定蓼子七中没食子酸含量
Content Determination of Gallic Acid in Antenoron Filiforme ( Thunb. ) by HPLC
刘红莉 1贾文江 1曹望弟1
作者信息
- 1. 陕西省商洛市药品检验所,陕西商洛 726000
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the content determination of gallic acid in Antenoron Filiforme ( Thunb. ) . Methods The chromatographic column was InertSusTain C18 column ( 250 mm × 4. 6 mm, 5 μm ) , the mobile phase was methanol-water-phosphoric ( 15:85:0. 085 ) with a flow rate was 1. 0 mL/min, the detection wavelength was 270 nm and the column temperature was 35℃. Results The linear ranges of gallic acid in Polygonum seven was 0. 1415-0. 8305 μg/mL ( R2=0. 9999 ) . The average recovery was 98. 35% and RSD was 0. 60%. Conclusion The method is simple, rapid, has good stability, and can be used for the con-tent determination of gallic acid in Antenoron Filiforme ( Thunb. ) .关键词
高效液相色谱法/蓼子七/没食子酸/含量测定Key words
HPLC/Antenoron Filiforme ( Thunb. )/gallic acid/content determination分类
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刘红莉,贾文江,曹望弟..高效液相色谱法测定蓼子七中没食子酸含量[J].中国药业,2017,26(5):32-34,3.
