中国药业2017，Vol.26Issue(6)：5-8,4.DOI:10.3969/j.issn.1006-4931.2017.06.002
柠檬桉叶总黄酮提取工艺及其清除自由基活性研究
Extraction Techniques and Free Radical Scavenging Activity of Total Flavonoids from the Leaves of Eucalyptus Citriodora
摘要
Abstract
Objective To study the optimum extraction process of total flavonoids from the leaves of Eucalyptus Citriodora,and to test its scavenging free radical activity. Methods The orthogonal experiment was used to investigate the effects of ethanol volume fraction,ex-traction times,ratio of material to liquid and extraction time on the process. The scavenging free radical activity test method was used to remove DPPH and ABTS free radicals as indicators,to evaluate the ability of total flavonoids from the leaves of Eucalyptus Citriodora to scavenge free radicals. Results The optimal extraction conditions of total flavonoids from the leaves of Eucalyptus Citriodora were as follows:60% alcohol as extraction solvent,extraction time 1 h,material to liquid ratio 1 :15,extraction 3 times. Under these conditions, the maximum extraction rate of total flavonoids from the leaves of Eucalyptus Citriodora was 0. 3127%. Conclusion The optimum ex-traction condition and purification experiment are very useful for further development of industrial Process,which may be helpful for fur-ther development of Eucalyptus Citriodora as health protection foods.关键词
柠檬桉叶/总黄酮/提取工艺/自由基Key words
Leaves of Eucalyptus Citriodora/total flavonoids/extraction/free radical分类
化学化工引用本文复制引用
林三清,周中流,杨红艳,夏敬民,杨桂珍,周良霞..柠檬桉叶总黄酮提取工艺及其清除自由基活性研究[J].中国药业,2017,26(6):5-8,4.基金项目
国家级星火计划项目[2015GA780041] （）
广东高校青年创新人才项目 （）
广东省高等学校优秀青年教师培养计划项目[YQ2015109]. （）
