中国药业2017，Vol.26Issue(6)：12-15,4.DOI:10.3969/j.issn.1006-4931.2017.06.004
丙泊酚与水合氯醛对亚低温大鼠海马神经元凋亡的影响比较
Effect Comparison of Propofol and Chloral Hydrate on the Apoptosis of Hippocampal Neurons in Mild Hypothermia Rats
摘要
Abstract
Objective To compare the intervention effect of Propofol and Chloral Hydrate on the apoptosis of nerve cells in mild hy-pothermia rats,in order to promote Propofol better applied in clinical hypothermic anesthesia surgery. Methods Totally 30 health SD rats were selected and randomly divided into normal temperature group of chloral hydrate(group A),low temperature group of propofol group(group B)and low temperature group of chloral hydrate(group C),10 cases in each group. The body temperature of group A was maintained normal,and those of group B and group C were under low-temperature intervention for 30 min,each group underwent heart perfusion,the brains were tahen after decapitation to prepare the rat hippocampal neuronal tissue samples,and then apoptosis marher Caspase-3 was detected and the ultra-microstructure was observed by transmission electron microscopy to compare the influence of Propofol and Chloral Hydrate on the apoptosis of nerve cells in mild hypothermia rats. Results The semi-quantification was carried out on the expressions of Caspase-3 protein by average optical density ( AOD ) . The result showed that the expression of Caspase-3 protein in group C was significantly lower than that in group A,but higher than that in group B (p < 0. 01);The results of the trans-mission electron microscopy showed that there were obvious apoptotic bodies formed in the hippocampal neurons in group A,while there were also different degrees of apoptotic tendency in group C,and the apoptosis phenomenon was only not serious in group B. Conclusion There are certain of apoptosis phenomena in the hippocampal neurons in hypothermia rats,while the Propofol can reduce the apoptosis of hippocampal neurons under hypochromic condition,and the Chloral Hydrate has no obvious intervention effect on the apoptosis of hippocampal neurons at the same condition.关键词
丙泊酚/水合氯醛/亚低温/海马神经元/凋亡Key words
Propofol/Chloral Hydrate/hypothermia/hippocampal neuron/apoptosis分类
医药卫生引用本文复制引用
何亚,刘晓颖,邓庆华,郑小红,蒋红艳,苏爱淇..丙泊酚与水合氯醛对亚低温大鼠海马神经元凋亡的影响比较[J].中国药业,2017,26(6):12-15,4.基金项目
重庆市教育委员会科学技术研究项目[KJ112502]. （）
