中国药业2017，Vol.26Issue(6)：28-30,3.DOI:10.3969/j.issn.1006-4931.2017.06.008
超高效液相色谱法测定消积化虫散中厚朴酚与和厚朴酚含量
Content Determination of Magnolol and Honokiol in Xiaoji Huachong Powder by UHPLC
潘伟 1谢扬 1杨雪峰 1揭定华1
作者信息
- 1. 重庆市永川食品药品检验所,重庆 402160
- 折叠
摘要
Abstract
Objective To establish a UHPLC method for the content determinion of magnolol and honohiol in Xiaoji Huachong Powder. Methods The experiment was performed with Thermo AccliamTM RSLC PA2 column(100 mm × 2. 1 mm,2. 2 μm). The mobile phase was methanol-water (70 :30,V/ V),the flow rate was 0. 3 mL/min,the column temperature was 35 °C,the detection wavelength was 294 nm,and the injection volume was 2 μL. Results The magnolol and honohiol showed a good linear relationship in range of 0. 0405-0. 1215 μg(r=0. 9997),0. 0101-0. 0608 μg(r=0. 9996),respectively. The average recoveries were 100. 05%,100. 15%,and RSDs were 0. 47%,1. 26%(n=6),respectively. Conclusion This method is simple and effective,which can be used to determinate the content of magnolol and honohiol in Xiaoji Huachong Powder.关键词
消积化虫散/超高效液相色谱/厚朴酚/和厚朴酚/含量测定Key words
Xiaoji Huachong Powder/UHPLC/magnolol/honohiol/content determination分类
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潘伟,谢扬,杨雪峰,揭定华..超高效液相色谱法测定消积化虫散中厚朴酚与和厚朴酚含量[J].中国药业,2017,26(6):28-30,3.
