中国中西医结合外科杂志2017，Vol.23Issue(1)：60-64,5.DOI:10.3969/j.issn.1007-6948.2017.01.017
桃核承气汤对大鼠糖尿病大血管Toll样受体表达的影响
The Effect of Tao-He-Cheng-Qi Decoction on Vascular Expression of Toll-like Receptor 2, Toll-like Receptor 4, Transforming Growth Fator-beta and Insulin-like Growth Fator-1 in Rats with Diabetes
摘要
Abstract
Objective To observe the effects of Tao-He-Cheng-Qi decoction on rat vascular expression of toll-like receptor-2 (TLR-2),TLR-4,transforming growth factor-beta (TGF β),and insulin-like growth factor-1 (IGF-1) expression.Methods Male SD rats were divided to a control group (A group,n=30),a model group (B group,n=31),an early-treatment group (C group,n=31) and a regular-treatment group (D group,n=31).Rats in A group had no drug intervention,but received 0.9％ sodium chloride (10 mL/kg) gavage everyday in rats of B group.Rats in C group were received Tao-He-Cheng-Qi decoction by gavage before and after diabetic modeling.The rats of D group were received Tao-He-Cheng-Qi decoction after diabetes modeling.After 1,12,or 20 weeks of intervention,rats were sacrificed.Immunohistochemical methods were used to detect the expression of TLR-2,TLR-4,TGFβ and IGF-1 in large blood vessel.Results Twenty weeks after intervention,the levels of TLR-2 expression were (9.333± 2.338) pg/mL in D group,but significantly decreased in C group (3.750 ± 1.282 pg/mL,P ＜ 0.05).The levels of TLR-2 expression were not different between D and B group.The levels of TL-4 expression were (3.750 ± 1.282) pg/mL in C group and (5.750± 1.982) pg/mL in D group,which was significantly decreased compared to that in B group (10.333 ± 2.658 pg/mL,P ＜ 0.05).The expression levels of TGF-beta was (403.438 ± 11.273) pmol/L in C group and (535.313±12.384) pmol/L in D group,with significant difference (P＜ 0.05).The expression levels of IGF-1 were (1104.375±55.495) pmol/L in C group and (861.000±28.656) pmol/L in D group.The difference was significant (P ＜ 0.05).Conclusion Tao-He-Cheng-Qi decoction can reduce diabetic vascular fibrosis,and also can prevent diabetic vascular fibrosis when early intervention.关键词
大血管纤维化/早期干预/Toll样受体/转化生长因子β/胰岛素样生长因子1Key words
Macrovascular fibrosis/early intervention/toll-like receptor/transforming growth factor-beta/insulin-like growth factor 1分类
生物科学引用本文复制引用
徐阳,王军..桃核承气汤对大鼠糖尿病大血管Toll样受体表达的影响[J].中国中西医结合外科杂志,2017,23(1):60-64,5.基金项目
国家自然科学基金面上项目(81173268) （81173268）
