中国中西医结合外科杂志2017，Vol.23Issue(1)：65-68,78,5.DOI:10.3969/j.issn.1007-6948.2017.01.018
基于脂质组学的高脂血症小鼠血清脂肪酸研究
Serum Fatty Acids of Hyperlipidemia Mice Based on Lipidomics
刘佩莉 1关鑫 2李楠 1曹兴奋 1杨萍 1王晓辉 1刘洪斌1
作者信息
- 1. 天津市医药科学研究所,天津300020
- 2. 天津市南开医院中西医结合急腹症研究所,天津300100
- 折叠
摘要
Abstract
Objective To investigate the serum fatty acids composition and biomarkers in hyperlipidemia mice using high performance liquid chromatography (LC-Q-TOF-MS) technique.Methods Total 20 mice were randomly divided into normal control group and model group.Hyperlipidemia model was established after intragastric administration once a day,continuously intragastric administration at the end of 15 days.One hour after the last administration,the blood was taken from inner canthus.Mter centrifuge extraction,samples were assayed by LC-Q-TOF-MS technology in negative mode.Data were analyzed by partial least squares (PLS-DA) of two groups of serum fatty acids composition differences.Results Compared with the control group,serum TG (1.037±0.393 vs 0.810±0.412),TC(3.776±0.821 vs 3.002±0.648),HDL-C(2.282±0.544 vs 1.896±0.326) and LDL-C (0.405±0.148 vs 0.128±0.072) levels in model group were significantly higher.Analysis of the precision RSD was 9.25％.The results showed 16 potential biomarkers.Conclusion Metabolic characteristics of the serum fatty acids components in hyperlipidemia mice were studied by LC-Q-TOF-MS technology.It could distinguish the hyperlipidemia model group and the control group.It was an effective pathway to find unknown biological markers.关键词
高脂血症/脂质组学/脂肪酸/生物标志物Key words
Hyperlipidemia/lipidomics/fatty acids/biological markers分类
生物科学引用本文复制引用
刘佩莉,关鑫,李楠,曹兴奋,杨萍,王晓辉,刘洪斌..基于脂质组学的高脂血症小鼠血清脂肪酸研究[J].中国中西医结合外科杂志,2017,23(1):65-68,78,5.
