中国中西医结合外科杂志2017，Vol.23Issue(2)：113-118,6.DOI:10.3969/j.issn.1007-6948.2017.02.003
柯萨奇病毒腺病毒受体在肺癌中的表达和意义
Coxsackie Adenovirus Receptor Expression in Lung Cancer and Its Significance
摘要
Abstract
Objective To investigate the expression of (Coxsackie virus adenovirus receptor,CAR) in nor-mal lung tissue, para-cancerous tissue and lung cancer tissues and the effect of CAR expression in vitro on pro-liferation,invasion and migration of human lung squamous cell carcinoma NCI-H520 cells. Methods Total 100 cases of lung cancer, adjacent tissues and normal lung tissues were collected. Immunohistochemistry, West-ern blot and RT-PCR were used to detect the expression of CAR protein and mRNA. RNAi technique was used to diminish CAR expression. The effects of RNAi on the proliferation, invasion and migration of NCI-H520 lung cancer cells were studied by plate cloning assay and transwell invasion and migration experiments. The animal model was used to study the growth of the cancer cells in vivo. Results Immunohistochemical results showed gene were screened. Semi-quantitative RT-PCR and Western blot showed that the expression of CAR mRNA and protein in these cells decreased in different degrees, and shRNA-2 had the strongest inhibitory effect. Plate clone formation assay showed that shRNA-2 inhibited the proliferation of NCI-H520 cells (P<0.05). Transwell invasion and migration experiments showed that CAR-inhibited NCI-H520 cells had a significant decrease in in-vasion and migration (P< 0.05). Animal model results show that following the inhibition of CAR expression, the growth of transplanted tumors in animals was inhibited slow. Conclusion CAR is associated with the develop-ment and progression of lung cancer and can promote the invasion and migration of lung cancer cells. Nude mice lung cancer transplanted tumor model was successfully established for the future study of lung cancer. The expression of CAR gene was effectively inhibited by RNAi, and, therefore, the growth of NCI-H520 cells in nude mice was inhibited. CAR was expected to be one of the targets of lung cancer gene therapy.关键词
柯萨奇病毒腺病毒受体/Westernblot/逆转录聚合酶链反应/平板克隆形成实验/移植瘤模型Key words
Coxsackie adenovirus receptor/western blot/RT-PCR/colony formation assay transwell experi-ments/transplanted tumor model分类
医药卫生引用本文复制引用
董元焕,孙蕾娜,战忠利..柯萨奇病毒腺病毒受体在肺癌中的表达和意义[J].中国中西医结合外科杂志,2017,23(2):113-118,6.基金项目
天津市卫生局局级基金项目(2010KZ11) （2010KZ11）
