摘要
Abstract
Objective To observe the intervention effects of Ronggan decoction in dimethylnitrosamin( DMN)-induced chronic hepatic fibrosis in rat. Methods A total of 35 Wister male rats were randomly divid-ed into the normal control group and the model group. The model group was used to establish the hepatic fibro-sis model with 0.5%DMN by intraperitoneal injection of 2 mL/kg of body mass for 4 weeks, and then by random-ly divided the rats into model group and Ronggan group. The two groups were treated with corresponding drug in-tragastrically every day for 3 weeks. Rats were then sacrificed for sampling at 7 the weekend, and the serum lev-els of alanine aminotransferase (ALT), aspartate aminotransferase (AST),albumin (Alb) , total bilirubin (TBIL), as well as hepatic levels of hydroxyproline (Hyp) were measured. Pathological changes of liver tissue was evaluat-ed by Sirius Red staining and HE staining. Collagen typeⅠ and smooth muscle actin (α-SMA) protein level in liver was determined by western blotting. Results Compared with the normal control group, serum levels of TBIL, ALT, AST were significant increased and Alb were significant descend in the model group(P<0.05). Af-ter the treatment of the Ronggan decoction, serological index of liver function improved significantly. HE stain-ing showed that the model group had a large number of inflammatory cell infiltration, swelling, degeneration and necrosis, and bleeding phenomenon was significant in the liver tissue. Sirius red staining showed that the liver collagen of the model group was significantly hyperplasia, forming a pseudo lobule, collagen area was significant-ly increased. The Hyp content of the liver tissue of the model group were significantly increased than the normal control group [(1412.42±367.68)μg/g VS(530.20±62.37)μg/g,P<0.01], and the expression levels of colla-gen type Ⅰand α-SMA has a significant increased. The degree of inflammation and fibrosis in liver tissue were improved in the model group after the drug intervention, and the Hyp content of the liver tissue were decreased obviously [(1092.83 ± 146.19)μg/g]. Statistical analysis shows that the liver tissue collagen area of Ronggan group were significantly descend than the model group [(358810±235058) ×104μm2 VS(358810±235058)× 104μm2, P<0.05], and the expression levels of collagen type Ⅰ antibodies and α-SMA were decreased obvi-ously. Conclusion Ronggan decoction has better reverse action to liver fibrosis induced by DMN.关键词
荣肝汤/肝纤维化/二甲基亚硝胺/生化指标/病理学变化Key words
Ronggan decoction/liver fibrosis/dimethylnitrosamine/biochemistry indexes/pathologic change分类
生物科学
